Gene Sequencing and Haemolysis of Bacteria in Clarias gariepinus From Ajilete Location on Yewa River
Journal Title: Annual Research & Review in Biology - Year 2016, Vol 10, Issue 4
Abstract
Gene sequencing and haemolysis of bacteria in Clarias gariepinus post juveniles sampled from Ajilete location on Yewa River, Ogun state, Nigeria was carried out. Bacteria were isolated from the gut, gills and skin of the fish. Thirty bacteria isolates were selected and the DNAs were extracted using hexadecyltrimethylammonium bromide (CTAB) method and Polymerase Chain Reaction (PCR) amplification of the isolates was carried out using universal primer for bacteria. The purification of PCR product was done using ethanol precipitation and thereafter the DNAs were sequenced using automated DNA sequencer. The sequence data were analyzed on the GenBank database (NCBI) using Basic Local Alignment Search Tool (BLAST) to find the nearest related sequence, and to construct the phylogenetic relationship. The haemolysis of the selected isolates were also carried out. Data collected were subjected to descriptive statistics. Nine genera of bacteria isolated were Escherichia, Staphylococcus, Enterobacter, Proteus, Pseudomonas, Klebsiella, Citrobacter, Streptococcus and Bacillus. The 16S rRNA gene sequencing method identified the isolates as strains of Brevibacillus agri, Bacillus cereus, Ignatzschineria indica, Comamonas kerstersii, Proteus vulgaris, Lysinibacillus sphaericus, Proteus hauseri, Clostridium bifermentans, Proteus mirabilis, Pseudomonas putida, Escherichia coli, Comamonas jiangduensis, Enterobacter cloacae, Serratia marcescens, Alcaligenes faecalis, Lysinibacillus fusiformis, Myroides odoratimimus. Haemolysis showed that M. odoratimimus, B. cereus, C. kerstersii, P. vulgaris, C. bifermentans, E. coli and E. cloacae all had β-haemolysis. A. faecalis displayed α-haemolysis while, B. agri, I. indica, L. sphaericus, P. hauseri, L. fusiformis, S. marcescens were non-haemolytic. This study confirms the reliability of the 16S rRNA gene sequencing method. This study also concludes that there are different pathogenic bacteria species that are associated with C. gariepinus.
Authors and Affiliations
O. O. Ololade, A. R. Oloyede, A. A. Idowu, Y. A. Babarinde
Keywords
Related Articles
- EP ID EP351072
- DOI 10.9734/ARRB/2016/26923
- Views 91
- Downloads 0
Platelets’ Functional Peculiarities in Persons of the Second Mature Age with Spinal Column Osteochondrosis of the Second Degree
Introduction: Many disturbances in a body are often accompanied by some disturbance of blood parameters. Regular blood elements and, especially, platelets can play a significant role in development of dystrophic changes...
Antibacterial Evaluation of Aqueous and Ethanol Extracts of Ocimum gratissimum and Carica papaya
Aims: This study was carried out to evaluate the antibacterial activity of aqueous and ethanol leaf extracts of Ocimum gratissimum and Carica papaya against selected clinical isolates of Gram-negative bacteria and to als...
ABO Blood Groups and Malaria Parasitaemia in Outpatients of BMSH Port Harcourt, Rivers State
A cross – sectional study was conducted to investigate the relationship between ABO blood groups and malaria parasitaemia in Port Harcourt, Rivers State, Nigeria. Thick and thin films made from venous blood samples colle...
Adhesion of Heterotrophic Bacteria on the Surface of Containers Used for Well Water Storage in Garoua (North Cameroon)
Aims: The abundance of heterotrophic bacteria adsorbed on the walls of four types (polypropylene, glass, aluminium, clay) of containers used for well water storage in Garoua was compared. Methodology: Water samples were...
Comparative Study of the Response of Four Native to the Bulgarian Black Sea Coast Psammophytes to Simulated Flooding Experiments
Aims: This study aimed 1) to determine and compare the effects of flooding stress, caused by storms on whole plants of four native to the Bulgarian Black Sea Coast psammophytes and how long their rhizomes can remain viab...