Genetic analysis of polyketide synthase and peptide synthase genes of cyanobacteria as a mining tool for new pharmaceutical compounds
Journal Title: Journal of Pharmaceutical & Health Sciences - Year 2017, Vol 5, Issue 2
Abstract
Cyanobacteria are considered a promising source for new pharmaceutical lead compounds and a large number of chemically diverse and bioactive metabolites have been obtained from cyanobacteria. Despite of several worldwide studies on prevalence of NRPSs and PKSs among the cyanobacteria, none of them included Iranian cyanobacteria of Kermanshah province. Therefore, the aim of this study was to amplify the NRPS and PKS genes. These stains were the most frequent species of that zone. The DNA sequences of the 16s rRNA region were determined and the strains were named as Scytonema sp. N11, Calothrix sp. N42 and terrestrial Nostoc SP. N66). Lastly, to show that the presence of these genes correlates with natural product synthesis, we conducted biochemical assays to detect the presence of antimicrobial effects in organic extracts. The results of these experiments suggest that both NRPS and PKS genes are presence in all three strains. Finally the sequences presented in this study have been deposited in GenBank for getting the accession numbers (NRPS A domains) and (PKS KS domains). According to these results, it is concluded that the antibiogram bioassay and molecular detection of peptide synthetase and polyketide synthase genes in cyanobacterial strains may be useful techniques for the assessment of natural product -producing species and possible role of peptide synthetase and polyketide synthase enzyme complexes in the biosynthesis of biologically active compounds.
Authors and Affiliations
Bahareh Nowruzi, Hossein Fahimi, Neda Ordodari, Reza Assareh
Keywords
Related Articles
- EP ID EP214895
- DOI -
- Views 117
- Downloads 0
Physicochemical Analysis of Margarine, Butter and Butter Oil Samples of Iran’s Markets
Butter, margarine and butter oil are three products which consume daily by Iranian community. Detection of type and existence of synthetic antioxidants (SAs) quantities in the margarine, butter, and butter...
The Effect of Hydro-alcoholic Extract of Leaves of Vitex on the Gestation Indices of Male Rats
Background: Phytoestrogens are some plant compounds with estrogenic biological effects which are found in many nutritional sources as soybean, flaxseed, and sesame. Vitex agnus-castus, also called Vitex, owns phytoestr...
The Efficiency of Bio-adsorption of Heavy Metals from Pharmaceutical Effluent by Rumex crispus L. Seed
The programs of managing waste materials in developing countries are often unsatisfactory and the unreasonable disposal of waste is a major issue in the worldwide. The main aim of the current study is to: Assess the appl...
Lactulose efficacy in reduction of nitrogen products, blood potassium and fluid overload in patients with end-stage renal failure
Introduction. Chronic kidney disease (CKD) is a major public health problem that often goes unrecognized until late-stage disease. Patients with chronic kidney disease (CKD) face with uremic toxins and hyperkalemia. Also...
Comparison the effect of carboxymethyle cellulose films containing Thymus vulgaris and Zataria multiflura on shelf life of chicken meat
Poultry meat is a kind of perishable food. The growth of pathogenic microorganisms may occur in chicken meat during storage in the refrigerator. Microbial growth causes a serious hazard to the safety of the food consumer...