Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt
Journal Title: Journal of Applied Veterinary Sciences - Year 2021, Vol 6, Issue 2
Abstract
Corynebacterium pseudotuberculosis is a small Gram-positive bacillus containing mycolic acid in the structure of the cell wall. The bacterium is responsible for Caseous Lymphadenitis (CLA) in small ruminants (sheep and goats). The bacteria are also responsible for Ulcerative Lymphangitis in equines. The disease causes great economic loss in the animal industry. This work aimed to check the ability of Quadruplex PCR (Q-PCR) for genotyping and identification of Egyptian isolates of C. pseudotuberculosis and sequence analysis of phospholipase D (PLD) gene of local isolates. Four of C. pseudotuberculosis local isolates previously biochemically identified were tested for narG gene (nitrate reductase gene). Both nitrate negative biovar (ovis) and nitrate positive biovar (equi) showed a positive result for 16S rRNA, rpoB and PLD genes of C. pseudotuberculosis species. The sequence analysis of our local isolates' PLD gene revealed minor changes in PLD proteins between ovine and equine strains compared with other published PLD sequences in GenBank. It was concluded that the Q-PCR method is able to differentiate between C. pseudotuberculosis equi and ovis biovars. Also, the sequence of PLD gene of local isolates representing the two biovars revealed some variation, which leads to an accurate diagnosis of C. pseudotuberculosis biovars and generates a mapping of immerged local isolates. Further, the PCR and sequence of these isolates provide rapid and accurate genotyping, especially with hyperimmune serum's unavailability.
Authors and Affiliations
Nadine A. El-Sebay, Marwah M. Mohamed, Elham F. El-Sergany, Ashraf M. Abbas, Roukaya M. Osman, Dalia A. M. Abd El-Moaty1
Keywords
Related Articles
- EP ID EP693783
- DOI https://dx.doi.org/10.21608/javs.2021.154576
- Views 130
- Downloads 0
Ultrasonography, Histopathological Udder Alterations And Bacteriological Investigations For Diagnosis Of Mastitic Goats
The purpose of this study was to evaluate the ultrasound and histopathological findings in comparison with the bacteriological examination to help in prognosis decision for udder alteration occurred by different cause an...
Antioxidant and Hepatoprotective Effects of Tranilast against Thioacetamide-Induced Liver Fibrosis in Rats
The aim of the present study was to investigate the potential antioxidant and Hepatoprotective activities of tranilast against TAA-induced liver fibrosis in rats. Methods: Liver fibrosis was induced in rats by intraperit...
Factors influencing dietary tannin inclusion in dairy diets: A review
T The objective of this paper was to evaluate factors affecting tannin dietary inclusion on enteric methane emission (CH4) and performance in dairy cows. Dairy production contributes to the greenhouse effect as it natura...
Detection of Mold and Aflatoxin B1 in Mayonnaise Product from Egyptian Markets by HPLC
Egyptian consumers' demand and preference for sauces like mayonnaise have increased lately. The processing and packaging techniques of such products may safeguard the consumer from bacterial hazards, although mold and/or...
Food Safety Programs that should be Implemented in Slaughterhouses: Review
Fresh meat and poultry meat are easily contaminated with various contaminants especially if it is not properly handled and preserved, leading to loss of quality and potential public health problems.Food safety addresses...