Genetic characterization of the domestic pig (Sus scrofa domestica) in Cerete-Colombia, using microsatellite markers
Journal Title: Revista MVZ Cordoba - Year 2014, Vol 19, Issue 2
Abstract
Objective. The purpose of this study was to characterize a population of domestic pig (Sus scrofa domestica) in Cereté, Córdoba, using 20 microsatellite; calculate heterozygosity per locus and average heterozygosity. Materials and methods. Hair samples were collected from 62 specimens. DNA was extracted by proteinase K digestion and phenol-chloroform purification. Information from 20 microsatellites was selected out of those recommended for swine biodiversity studies. PCR products were separated by a vertical polyacrylamide gel electrophoresis. The bands were visualized by staining with silver nitrate. Results. All microsatellites used were polymorphic. Between 3 (SW1067) and 15 (IFNG) alleles were detected with an average number of 6.7 and a total de 134 alleles. The average expected and observed heterozygosities were 0.5278 and 0.5479, respectively. PIC values ranged between 0.1999 and 0.8300 for loci SW1067 and SW911, respectively. Conclusions. Levels of observed and expected heterozygosity found in the present study indicate that the domestic pig (Sus scrofa domestica) in Córdoba Cereté show high degree of genetic variability.
Authors and Affiliations
Iván Meléndez G, M. Sc *| Universidad de Pamplona. Faculty of Basic Sciences. Department of Biology. Laboratory of Genetics. Ciudad Universitaria. Pamplona, Colombia.Correspondence: imgelvez@hotmail.com, Enrique Pardo P, Ph. D| Universidad de Córdoba. Department of Biology. Genetics Section. Carrera 6 No. 76-103. Monteria. Colombia., Teodora Cavadia M, M. Sc| Universidad de Córdoba. Department of Biology. Genetics Section. Carrera 6 No. 76-103. Monteria. Colombia.
Keywords
Related Articles
- EP ID EP3362
- DOI -
- Views 373
- Downloads 20
Roadkill hotspots in a protected area of Cerrado in Brazil: planning actions to conservation
Objective. Here we aimed to identify the main points of animal death by roadkill in the view of helping mitigation plans and reducing the impact over the local fauna of a protected area. Materials and methods. We surveye...
Anti-leptospiral agglutinins in marmosets (Saguinus oedipus and Saguinus leucopus) from illegal trade
Objective. Determine the infection status with pathogenic Leptospira of one Saguinus oedipus and nine Saguinus leucopus at the Cali Zoo that had been confiscated in Colombia from illegal trade. Materials and methods. A f...
SYNTHESIS, COMPOSITION AND MODIFICATION OF THE BOVINE MILK FAT: A valuable nutrient for the human health
It presents a review about the composition, structure and mechanism of milk fat synthesis in the bovine mammary gland, and about the effect of unsaturated fatty acid (UFA) supplementation on the amount and quality of mil...
EFECTO DEL MEDIO Y DE LA HERENCIA SOBRE LOS PESOS AL NACER, DESTETE Y 16 MESES DE EDAD EN LA RAZA COSTEÑO CON CUERNOS
With the objective to estimate genetics parameters for the productive characters, weights upon newborn, weaning and 16 months of age, in the race «Costeño con cuernos» at the Turipaná experiment station at Cereté city, a...
Evaluation of three bore leaf meal (Alocasia macrorrhiza) diets in broilers
Objective. Assessing the nutritional impact of including bore leaf meal in proportions of 0 (T1), 5 (T2), 10 (T3) and 15% (T4) in the supply of 100 male chickens of the Ross 308 line. Materials and Methods. A completely...