Genetic distinction among cultivars of date palm using some molecular markers
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2014, Vol 4, Issue 1
Abstract
The experiment was carried out in the laboratories of biological techniques in the Department of Livestock, Agriculture College, Basrah University. The Randomly Amplified Polymerase Chain Reaction technique was used with four primers to find out the exactration DNA and the percentage of genetic divergence in six cultivars of date palm (Al_khasab, Allylwi,Bent Alsabaa, Dekal Ahmer, Alfersee and AlKhdrawee).Also, the study involved isolating DNA from the newly grown leaves of the study's date palm cultivars, and showing differences among the amplified pieces for each cultivar by deporting samples to the Electrophorses in the RAPD interactions .The results stated that there are considerable genetic differences among the cultivars of the current study. This was in terms of both the number of amplified bands and their molecular weight according to the primer used. The total number of the bands used was 64 for all primers. Moreover, the cultivars showed huge variations except ,Bent Alsabaa and Dekal Ahmer which stood at 75% and Dekal Ahmer and AlKhdrawee stood at 66.7%.The data was then collected and analyses, and the cultivars were classified into two separate groups. The cultivars Dekal Ahmer and Allylwi were in the same group where the former recorded less distance. Finally, the study concluded that the types are separated from each other reflected by phenotypic differences among them.
Authors and Affiliations
Abdul Kareem Mohammed Abd
Keywords
Related Articles
- EP ID EP654428
- DOI 10.24297/jab.v4i1.1948
- Views 218
- Downloads 0
The Geomagnetic Field Affect the Manifestation of Activity of Synthetic Biologically Active Substances.
We compared the influence of aqueous solutions of plant growth regulator (PGR) melaphen (MF), made under normal conditions and in conditions of low level electromagnetic field of the Earth (in permalloy container)...
Investigation the different ratios of carrier material to protect carotenoids in Gac (Momordica cochinchinensis Spreng) powder in drying process
Momordica cochinchinensis Spreng, Cucurbitaceae, is indigenous to Southeast Asia and consumed there for dietary as well as medicinal uses. In Viet Nam, this plant is called Ga, and the seed membrane (seed pulp or aril) o...
Effect of Ground Seeds of Picralima nitida Incorporated Diets on Serum Lipid Profile of Albino Rats
The effect of ground seeds of Picralima nitida incorporated diets on serum lipid profile of albino rats was studied. Twenty five albino rats randomly distributed into five groups of five rats were fed with 5%, 10%, 20% a...
STUDY OF THYROID DISTURBANCE IN DIABETIC AND NON-DIABETIC CHRONIC KIDNEY DISEASE PATIENTS
Objective: study the prevelance of thyroid disturbance in diabetic chronic kidney disease (CKD) patients and non-diabetic CKD patients. Background: CKD has been known to affect the pituitary-thyroid axis and the peripher...
P53 TRANSCRIPTION FACTOR AND DIABETES: IS THERE ANY LINK?
Diabetes, a metabolic disorder is alarmingly increasing across the globe and currently ample of research is being carried out to treat hyperglycaemia and its secondary complications in diabetic patients. Despite extensiv...