Genetic diversity of barley verities by RAPD markers
Journal Title: Agricultural Advances - Year 2013, Vol 2, Issue 12
Abstract
The fourteen barleyverities were studied by RAPD markersto assessment of their genetic diversity. From 40 used primers, 17 primersestablished suitable banding patterns. Totally, 435 bands were produced between564- 4157 bp that all bands were polymorphic in varieties. The total mean ofmarkers for each primer was 25.59 and mean of polymorphic markers for eachprimer and variety were 27.9 and 22.29, respectively. “Gara Arapa” with 104 and“Gorgan” with 64 bands had the highest and lowest polymorphic bands,respectively. Primer 7 had the highest polymorphic band (35) and primer 11 hadthe lowest polymorphic band (13). Polymorphic information content (PIC) forprimers was estimated among 0.26 (Primer 2) and 0.48 (Primer 13) with mean0.34. Marker index (MI) varied among 2.01 to 8.97 in primers 2 and 7,respectively. Nei’s gene variation index was estimated 0.288for RAPD markers data. These amounts showed partly high diversity among understudied varieties. The genetic distance varied from 0.2509 between “Gara Arapa”and “Dari-Friz 88-A1” to 0.7135 between “Gohar Jo” and Dari-Friz 88- A7. TheCluster analysis based on Nei's genetic distance classified genotypes to threegroups. The first group contains Gorgan, Makui, Goharjo and Dari-Friz88-A1. Thesecond group contains Zarjo, Reihan, Karoon, Garearpa, Sahand, Valfajr andDari-Friz88-A7. The third group consists of Sina, Dari-Friz88-A3 andDari-Friz88-A5.
Authors and Affiliations
A. Pourmohammad*| Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran., D. Mahdavisafa| Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.
Keywords
Related Articles
- EP ID EP530
- DOI 10.14196/aa.v2i12.1113
- Views 485
- Downloads 26
Determinants of loan repayment of livestock farmers under agricultural credit guarantee scheme (A.C.G.S) in Etche local
This study examined the determinants of loan repayment of livestock farmers under the Agricultural Credit Guarantee Scheme (ACGS) in Etche Local Government Area of Rivers State, Nigeria. Simple random sampling techniq...
Allelopathic effect of pistacia khinjuk leaf extracts on chenopodium album, physalis alkekengi and amaranthus retroflexu
A weed is a term applied to any plant considered as one major problem in farm fields and gardens. Weeds cause serious damages to farmers per year, bringing about heavy losses in farm and garden yield. Thus, in order to...
Optimizing the potassium (K) dose of fertigation for the Nagpur mandarin (Citrus reticulate Blanco)
To standardize the potassium sulphate fertilizer dosethrough fertigation system during three Ambia flowering (October flush) onyield and quality of 14-16 years Nagpur mandarin a field experiment wasconducted at Nationa...
Kinetics of biogas production from jackfruit wastes co-digested with cow paunch in batch mode
Application of appropriate mathematical models is one of the strategies in solving the stability problems often exhibited by anaerobic digestion process. Kinetics of batch anaerobic digestion of jackfruit waste co-dige...
Prevalence of helminth parasites in Gallus domesticus from Gurez valley
During the study period of two years the prevalence of helminth parasites in domestic fowl from Gurez valley were analysed. A total of 137 domestic fowl were examined for helminth parasites from May 2013 to April 2015....