Genetic diversity of barley verities by RAPD markers
Journal Title: Agricultural Advances - Year 2013, Vol 2, Issue 12
Abstract
The fourteen barleyverities were studied by RAPD markersto assessment of their genetic diversity. From 40 used primers, 17 primersestablished suitable banding patterns. Totally, 435 bands were produced between564- 4157 bp that all bands were polymorphic in varieties. The total mean ofmarkers for each primer was 25.59 and mean of polymorphic markers for eachprimer and variety were 27.9 and 22.29, respectively. “Gara Arapa” with 104 and“Gorgan” with 64 bands had the highest and lowest polymorphic bands,respectively. Primer 7 had the highest polymorphic band (35) and primer 11 hadthe lowest polymorphic band (13). Polymorphic information content (PIC) forprimers was estimated among 0.26 (Primer 2) and 0.48 (Primer 13) with mean0.34. Marker index (MI) varied among 2.01 to 8.97 in primers 2 and 7,respectively. Nei’s gene variation index was estimated 0.288for RAPD markers data. These amounts showed partly high diversity among understudied varieties. The genetic distance varied from 0.2509 between “Gara Arapa”and “Dari-Friz 88-A1” to 0.7135 between “Gohar Jo” and Dari-Friz 88- A7. TheCluster analysis based on Nei's genetic distance classified genotypes to threegroups. The first group contains Gorgan, Makui, Goharjo and Dari-Friz88-A1. Thesecond group contains Zarjo, Reihan, Karoon, Garearpa, Sahand, Valfajr andDari-Friz88-A7. The third group consists of Sina, Dari-Friz88-A3 andDari-Friz88-A5.
Authors and Affiliations
A. Pourmohammad*| Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran., D. Mahdavisafa| Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.
Keywords
Related Articles
- EP ID EP530
- DOI 10.14196/aa.v2i12.1113
- Views 481
- Downloads 26
Effect of different sowing dates and row spacing on the growth, seed yield and quality of off-season pea (Pisum sativum
A study was conducted to check the effect of different sowing dates and row spacing on the growth, seed yield and quality of off-season pea (Pisum sativum L. cv. Climax). Significant differences were recorded among sow...
Gerbera: Soil fertility and plant nutrition
Gerbera (Gerbera jamesonii Bolus ex. Hook) belonging to family Asteraceae is animportant cut flower, native to tropical Asia and Africa. Thumping success of Gerbera under protectedconditions has extensively discourage...
Productivity of taggar goats as affected by sex of kids and litter size
The experiment was conducted in Taggar goats to evaluate the effect of two different levels of protein and energy in ration on some reproductive and productive traits. Sixty two (62) kids of Taggar goats were used in t...
Influence of temperature humidity index on skin temperature of West African Dwarf goats raised in Nigeria
The influence oftemperature humidity index (THI) on skin temperature of West African Dwarf(WAD) goats was evaluated. Based on the recordings of farm ambient temperatureand relative humidity, the study was conducted dur...
Status and challenges of smallholder dairy cattle production in the highlands of Bale, Southeast Ethiopia
A diagnostic survey was conducted in the highlands of Bale, Southeast Ethiopia with the objectives to assess the status and challenges of smallholder dairy cattle production. The study area was stratified into two base...