Genetic Diversity of Curcuma in Thailand using External Transcribed Spacer (ETS) Sequences
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 5
Abstract
The Curcuma is the largest genus of Zingiberaceae which has been widely used as an important medicinal plants, spices and cosmetics. Consequently, genetic diversity from 22 samples of Curcuma was analyzed using the 5′ region of the external transcribed spacer (ETS) of 18S–26S nuclear ribosomal DNA sequences. The DNA sequences varied in length from 579-583bp. Neighbor-Joining (NJ) with Kimura -2-parameter was used to reconstruct phylogenetic trees. The genetic distance among samples ranged from 0.003-0.134. Curcuma was monophyletic entities based on ETS analyses. The phylogenetic tree showed that 22 samples of Curcuma could be separated into 2 groups with high bootstrap support. The most genetic similarity was found between C. aeruginosa and Curcuma sp. 1 which is consistent with the geographical distribution. The high bootstrap support (97%) indicated that Curcuma sp. 5, Curcuma sp. 6, Curcuma sp. 7, C. amada 1, C. amada 2, C. amada 3 and C. amada 4 were closely related species. This study represented the potential of ETS as a good candidate region for phylogenetic analysis of Curcuma species.
Authors and Affiliations
Pattamon Sangin, Preeyanit Mongkholsathian
Keywords
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