Genetic Diversity of Dry Bean (Phaseolus vulgaris L.) Accessions of Kenya Using SSR Markers
Journal Title: Journal of Experimental Agriculture International - Year 2015, Vol 5, Issue 4
Abstract
Aims: To determine the genetic diversity existing within the Kenyan dry bean using SSR markers. Place and Duration of Study: This study was conducted in Western Kenya and Bangor University, North Wales, between September 2010 and December 2012. Methodology: Thirty five (35) marketable dry bean samples collected from farmers, market centers as well as seed stockists were subjected to SSR analysis. Data generated was subjected to analysis with the GenAlEx 6.4 software assuming Hardy-Weinberg equilibrium to determine gene diversity index, number of polymorphic loci and alleles, genetic distances, analysis of molecular variance (AMOVA) and principal components analysis (PCA). NYTS-pc 2.1 software was used to construct an unweighted pair group method arithmetic averages (UPGMA) dendogram using the generated similarity coefficients. Results: Of the 7 SSR primers tested, 5 SSR primers were found to be polymorphic and used to screen the bean samples. The 5 primer combinations generated 49 polymorphic bands in 35 samples. Analysis of molecular variance accredited 8% of the disparity to diversity among the populations while the majority of the diversity (92%), resided within populations. The gene diversity index ranged from 0.1267 in the market population to 0.2377 in the Western province population. The highlands of Eastern province had a gene diversity index of 0.1475 while the dry lands had 0.1991. Cluster analysis segregated the bean samples into 9 clusters. Conclusion: There exists considerable variation in the dry bean of Kenya that is narrowing. There is need to intensify efforts to broaden the bean variation for sustainability. The population genetics of dry beans of Kenya are a possible guide to future bean breeding and germplasm management in Kenya.
Authors and Affiliations
Nyakio Kithinji Maryrose, Katherine Andrea Steele, Valerie A. P. Palapala
Keywords
Related Articles
- EP ID EP353847
- DOI 10.9734/AJEA/2015/10284
- Views 85
- Downloads 0
Some Okra Production Decisions and Farmers’ Awareness of Meloidogyne species Infection in Two Agro-ecologies, Ghana
A survey was conducted among okra farmers in two agro-ecologies (forest guinea savanna transition and moist semi-deciduous forest) of Ghana between August and December 2014, to assess their production decisions and aware...
Changes in Physicochemical Parameters during Storage of the Inflorescence Sap Derived from Four Coconut (Cocos nucifera L.) Varieties in Côte d’Ivoire
Aim: The study investigated the changes in the main physicochemical parameters of the coconut inflorescence sap derived from MYD, WAT, PB 121+ and PB 113+ varieties during storage. Place and Duration: Marc Delorme Resea...
Association of Stability Models in Measuring Stability of Common Bean Varieties
Plant breeders have been challenged with genotype by environment (G x E) interaction to develop high yielder and stable varieties. They have been using different univariate stability models to simplify the challenge. The...
Carcass, Organs and Economic Evaluation of Broiler Birds Fed Low-protein Diets Supplemented with the Most Limiting Essential Amino Acids in Ideal Protein Concept
This study evaluated the carcass, organs and economic parameters of broiler birds on the 57th day of production considered to be the economic market weight in which ideal protein concept was tested using the most limitin...
Production and Qualitative Aspects of Tomato Fruits under Leaf Fertilizer Applications with Resistance Bioinducers
The tomato is one of the cultures of great interest to the global agribusiness, especially to meet the industrial demand, resulting in income and quality of life to the producer. Given the complexity of culture, new tech...