Genetic diversity of six populations of red hybrid tilapia, using microsatellites genetic markers
Journal Title: Revista MVZ Cordoba - Year 2011, Vol 16, Issue 2
Abstract
Objective. To determine and evaluate the genetic diversity of six populations of red hybrid tilapia, with the purpose to assess the potential benefit of a future breeding program conducted at the Research Center for Aquaculture (Ceniacua), Colombia. Material and methods. A total of 300 individuals, representing a wide genetic variability, were genotyped using a fluorescent microsatellite marker set of 5 gene-based SSRs in 6 different farms belonging to 4 States of Colombia. Results. The result showed that the mean number of alleles per locus per population was 8.367. The population 5 had the highest mean number of alleles with 9.6 alleles, followed by population 4 with 9.4 alleles, population 2 with 9.2, population 3 with 8.0, population 1 with 7.2 and population 6 with 6.8 alleles. The analysis of the distribution of genetic variation was (17.32%) among population, while among individuals within populations was (28.55%) and within individuals was high (54.12%). The standard diversity indices showed that population 4 was the more variable (mean He=0.837) followed by population 1 (mean He=0.728), population 3 (mean He=0.721), population 5 (mean He=0.705), population 2 (mean He=0.690), population 6 (mean He=0.586). Highly significant deviations from Hardy–Weinberg, exhibited all of the populations, mostly due to deficits of heterozygotes. Genotype frequencies at loci UNH 106 of population 5 and loci UNH 172 of population 6 were Hardy-Weinberg equilibrium (HWE). Conclusions. The results of this study, contribute to the genetic breeding program of Tilapia, conduced by the Research Center for Aquaculture. The Fst distance showed that the samples are differentiated genetically and it is possible to use at the beginning of the genetic program. However, it is recommended to introduce others individuals to the crossbreeding program.
Authors and Affiliations
Boris Briñez R, M. Sc *| Centro de Investigaciones para la Acuacultura de Colombia (Ceniacua), Cra 9 C No. 114 – 60, Bogotá, Colombia.State University of Campinas – UNICAMP, Department of Genetics, Evolution and Bioagents. Institute of Biology, Brazil.Correspondencia:borisbrinez@hotmail.com, Xenia Caraballo O, M. Sc| Centro de Investigaciones para la Acuacultura de Colombia (Ceniacua), Cra 9 C No. 114 – 60, Bogotá, Colombia., Marcela Salazar V, MD| Centro de Investigaciones para la Acuacultura de Colombia (Ceniacua), Cra 9 C No. 114 – 60, Bogotá, Colombia.
THE INFLUENCE OF TEMPERATURE ON THE FEEDING BEHAVIOR OF Peltocephalus dumerilianus (TESTUDINES PODOCNEMIDAE)
Objective. To analyze the feeding behavior of 50 Peltocephalus dumerilianus hatchlings (Testudines Podocnemididae) under different temperature conditions in laboratory. Materials and methods. The individuals for this exp...
Prevalence of Listeria monocytogenes in pork-meat and other processed products from the Colombian swine industry
Objective. To determine the prevalence of L. monocytogenes in pork carcasses, meat cuts, and meat products (“chorizo”, sausage and ham). Materials and methods. Stratified sampling was implemented in meat-processed produc...
Seed removal by rodents in a fragment of dry tropical forest (Risaralda-Colombia)
Objectives. Rodents are the biggest seed predators in Neotropical ecosystems, however, fragmentation affects their presence, therefore seed predation. Materials and methods. The percentage and removal rate of seeds by ro...
Collection and evaluation of semen from the Capybara Hydrochoerus hydrochaeris
Objetive. Determine the characteristics of semen and morphometry of the Capybara (Hydrochoerus hydrochaeris) spermatozoid. Materials and methods. 10 males, weighing between 21-45 kg, which were restrained and anesthetize...
Steatosis in donkey (Equus asinus). First report in Colombia
A case of steatosis in a, 15 years old gelding donkey (Equus asinus), from the town of San Antero (Cordoba, Colombia) is described. the donkey, showed a hard and painful thickened nuchal ligament, giving the impression o...