Genetic diversity of six populations of red hybrid tilapia, using microsatellites genetic markers
Journal Title: Revista MVZ Cordoba - Year 2011, Vol 16, Issue 2
Abstract
Objective. To determine and evaluate the genetic diversity of six populations of red hybrid tilapia, with the purpose to assess the potential benefit of a future breeding program conducted at the Research Center for Aquaculture (Ceniacua), Colombia. Material and methods. A total of 300 individuals, representing a wide genetic variability, were genotyped using a fluorescent microsatellite marker set of 5 gene-based SSRs in 6 different farms belonging to 4 States of Colombia. Results. The result showed that the mean number of alleles per locus per population was 8.367. The population 5 had the highest mean number of alleles with 9.6 alleles, followed by population 4 with 9.4 alleles, population 2 with 9.2, population 3 with 8.0, population 1 with 7.2 and population 6 with 6.8 alleles. The analysis of the distribution of genetic variation was (17.32%) among population, while among individuals within populations was (28.55%) and within individuals was high (54.12%). The standard diversity indices showed that population 4 was the more variable (mean He=0.837) followed by population 1 (mean He=0.728), population 3 (mean He=0.721), population 5 (mean He=0.705), population 2 (mean He=0.690), population 6 (mean He=0.586). Highly significant deviations from Hardy–Weinberg, exhibited all of the populations, mostly due to deficits of heterozygotes. Genotype frequencies at loci UNH 106 of population 5 and loci UNH 172 of population 6 were Hardy-Weinberg equilibrium (HWE). Conclusions. The results of this study, contribute to the genetic breeding program of Tilapia, conduced by the Research Center for Aquaculture. The Fst distance showed that the samples are differentiated genetically and it is possible to use at the beginning of the genetic program. However, it is recommended to introduce others individuals to the crossbreeding program.
Authors and Affiliations
Boris Briñez R, M. Sc *| Centro de Investigaciones para la Acuacultura de Colombia (Ceniacua), Cra 9 C No. 114 – 60, Bogotá, Colombia.State University of Campinas – UNICAMP, Department of Genetics, Evolution and Bioagents. Institute of Biology, Brazil.Correspondencia:borisbrinez@hotmail.com, Xenia Caraballo O, M. Sc| Centro de Investigaciones para la Acuacultura de Colombia (Ceniacua), Cra 9 C No. 114 – 60, Bogotá, Colombia., Marcela Salazar V, MD| Centro de Investigaciones para la Acuacultura de Colombia (Ceniacua), Cra 9 C No. 114 – 60, Bogotá, Colombia.
Keywords
Related Articles
- EP ID EP3197
- DOI -
- Views 431
- Downloads 19
Roadkill hotspots in a protected area of Cerrado in Brazil: planning actions to conservation
Objective. Here we aimed to identify the main points of animal death by roadkill in the view of helping mitigation plans and reducing the impact over the local fauna of a protected area. Materials and methods. We surveye...
Activation and comparative kinetics of Ovis aries plasmin with human plasmin
Objective. Compare the kinetics and activations, unify the purifications methods and establish the sequence of the N-terminals of the plasminogens human and Ovis aries. Materials and methods. The plasminogens were purifi...
DIAGNOSTIC OF BULLS REPRODUCTION CAPABILITY FROM THREE LIVESTOCK FARMS OF THE UPPER MAGDALENA
The aim of this study was to evaluate the reproductive functionality of the bulls in the colombian region “Alto Magdalena”, analyzing the effect of age and breed type on semen quality and other important reproductive c...
Aflatoxicosis in chinchillas (Chinchilla lanigera)
Objetive. To determine the effects of dietary aflatoxins (AF) on chinchillas (Chinchilla lanigera). Materials and methods. A total of 20 male chinchillas were distributed in 5 experimental groups of 4 animals each. Anima...
Behavior of the Hydrochoerus hydrochaeris isthmius in a productive system, department of Córdoba, Colombia
Objective. To describe the behavior, of the subspecies H. hydrochaeris isthmius, during the dry and rainy seasons in catlle farms, Valencia, Córdoba, Colombia. Materials and methods. A population of animals between Septe...