Genetic Diversity Studies and Sensitivity to Antibiotics of Bacteria in Different Parts of Advanced Juveniles Clarias gariepinus from Idogo on Yewa River
Journal Title: Scholars Journal of Agriculture and Veterinary Sciences - Year 2016, Vol 3, Issue 3
Abstract
The study was conducted to determine the genetic relationship of the bacteria isolated from different parts of Clarias gariepinus using Random Amplified Polymorphic DNA (RAPD). PCR analysis as well as the antibiotics resistance profile was done. Bacteria isolates were taken from the skin, gills and gut of C. gariepinus. DNA was extraction from the bacteria isolates using CTAB method. The DNA extracted was subjected to RAPD-PCR analysis using five (5) RAPD primers. The RAPD gels were scored, analyzed using NTSYS program and the phylogenetic trees were constructed. The antibiotics resistance profile of these bacterial isolated were determined using disc diffusion method. Twenty bacteria species were isolated from the gill, gut and the skin of the Clarias gariepinus include Citrobacter murlinae strain, Escherichia coli strain, Morganella morganii strain, Alkaligenes feacalis strain, Citrobacter freundii, Citrobacter freundii strain, Citrobacter freundii strain, Morganella morganii stain, Erwinia tasmaniensis stain, Morganella morganii strain, Akaligenes feacalis strain, Proteus penneri strain, Citrobacter braaki strain, Morganella morganii strain, Citrobacter amalanoticus strain and Citrobacter freundii stain. The study revealed a genetic diversity which is required for populations to be more adaptive with the environmental changes. Eight (8) antibiotic substances were examined for the susceptibility of the species. These include Ceftazidime, Cefuroxime, Gentamicin, Ofloxacin, Augumentin, Nitrofurantion, Ciprofloxacin, Cefixime. However, Ciprofloxacin and Ofloxacin were found to be more effective as bacteria species were more resistance to other antibiotics. Keywords: Clarias gariepinus, Random Amplified Polymorphic DNA (RAPD), Clarias gariepinus
Authors and Affiliations
A A. Akinyemi ,, O. O. Oyelakin ,, A. R. Oloyede, J. K. Ekelemu ,, T. O. Aluko
Keywords
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