Genetic Diversity Studies of Bacteria Associated with Brycinus longipinnis from Eggua on Yewa River using Random Amplified Polymorphic DNA (RAPD) Markers
Journal Title: Scholars Journal of Agriculture and Veterinary Sciences - Year 2016, Vol 3, Issue 3
Abstract
Twenty samples of Brycinus longipinnis were collected from Eggua landing site on Yewa river Ogun state, which lies on latitude 7o03ˈN and longitude 2o55ˈE. The water quality parameters (Temperature, Dissolve Oxygen and pH), weight and the morphometric characteristics (Total Length, Standard Length and Head Length) were taken. The bacteria were isolated from gills, gut and skin of Brycinus longipinnis and characterized. The genomic DNA of the bacterial isolates were extracted and subjected to Random Amplified Polymorphic DNA RAPD using five Oligonucleotide primers to determine the genetic relatedness. The bands obtained were scored and the data were analyzed using NTSYS software to draw the dendogram. Haemolysis was also done. The results of the water quality parameters (Temperature, Dissolved Oxygen and pH) values were 28.6°C, 5.51ppm and 8.30 respectively. The mean values of the Total Length, Standard Length and Head Length were 5.60±0.2, 4.60±0.2 and 1.00±0.1 respectively. Fifty seven polymorphic markers and 10 monomorphic markers were generated from the five RAPD markers used. The dendogram showed that the bacterial isolates were similar to 66% thereafter, they split into two major groups, and Alcaligenes faecalis is the only bacterial isolate that is genetically different from the others. Proteus mirabilis and Proteus vulgaris were the only bacterial isolates that were genetically similar. Haemolysis shows that 35% of the isolates had α-haemolysis (partial haemolysis or incomplete haemolysis), 20% had β-haemolysis (complete haemolysis) and 45% had no haemolysis. Keywords: Brycinus longipinnis, Bacterial isolates, DNA extraction, RAPD, Haemolysis
Authors and Affiliations
A. R. Oloyede, O. O. Oyelakin, A. A. Akinyemi, J. K. Ekelemu, O. B Onireti, A. Quadri ,
Keywords
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