Genetic Identification of Siam Madu and Satsuma Mandarin Somatic Hybrid Genotypes Using SSR Markers
Journal Title: Scholars Journal of Agriculture and Veterinary Sciences - Year 2014, Vol 1, Issue 2
Abstract
Abstract: Simple sequence repeat (SSR) analysis was used to identify the genetic status 23 citrus somatic hybrid genotypes from Siam Madu +Satsuma Mandarin and their similarity to the parental. Five SSR primer pairs could amplify polymorphic SSRs from all of these genotypes. A total of 17 alleles with an average of 3.4 alleles per locus were detected. The polymorphic information content (PIC) among genotypes varied from 0.076 (TC26 285) to 0.673 (AG14). Pairwise coefficients of genetic similarity between all genotypes ranged from 0.24 to 1.00. Unweighted pair-group method arithmetic average (UPGMA) analysis allocated the genotypes and their parents in 4 major clusters. Cluster I, consists of 6 genotypes and Siam Madu with 89% genetic similarity Cluster II consist of 14 genotypes with 91% genetic similarity. Cluster III consist of F17 and cluster IV consist of Satsuma Mandarin, with 58,6% and 24% genetic similarity, respectively. The genetic distance between all somatic hybrid genotypes with the parental Satsuma Mandarin is 0.60, except for F17 the genetic similarity is 0.34. Keywords: Genetic, somatic hybrid, SSR markers, Siam Madu, Satsuma Mandarin.
Authors and Affiliations
C. Martasari, S. Ashari, L. Sulistyowati, A. Husni
Keywords
Related Articles
- EP ID EP380351
- DOI -
- Views 183
- Downloads 0
Intra-Specific Genetic Variability and Relationships between Grain Yield and Its Components in Pearl Millet (Pennisetumglaucum (L.) R. Br.) Genotype HKP Affected by End Cycle Drought in Niger
Abstract:Experimentation took place during the 2009 rainy season on the experimental station of the Institute of Radio-Isotopes of the University of Niamey in Niger (latitude 13°29' N, longitude 2°10' E). The objective o...
Increasing Agricultural Production and Productivity among Smallholder Farmers in Uganda through Agricultural Extension
Abstract: Agricultural extension offers an opportunity for increasing production without any significant quantitative increases in the basic factors of production: land, labour and capital. In Uganda, agricultural extens...
Effect of dietary inclusion of browse forage (Ziziphus mucronata) in a total mixed ration on performance of Yankasa rams.
An experiment was conducted to determine the feed value of Ziziphus mucronata forage in a total mixed ration.. Sixteen Yankasa rams weighing on the average 18.19±0.26kg were divided into four groups with four animals per...
Adoption level of Farm Machines by Crop Farmers in Delta State, Nigeria
The study delved into the examination of the adoption level of farm machines by crops’ farmers in Delta State, Nigeria. The specific objectives were to: describe the socioeconomic characteristics of the respondents; asc...
Effect of Garlic Powder (Allium sativum) Supplement on Blood Chemistry of West African Dwarf Rams
This study was designed to evaluate the effects of supplementing ruminant diets with garlic powder as source of natural feed additives on blood chemistry in sheep. Forty West African Dwarf (WAD) rams with average live we...