Genetic Identification of Siam Madu and Satsuma Mandarin Somatic Hybrid Genotypes Using SSR Markers
Journal Title: Scholars Journal of Agriculture and Veterinary Sciences - Year 2014, Vol 1, Issue 2
Abstract
Abstract: Simple sequence repeat (SSR) analysis was used to identify the genetic status 23 citrus somatic hybrid genotypes from Siam Madu +Satsuma Mandarin and their similarity to the parental. Five SSR primer pairs could amplify polymorphic SSRs from all of these genotypes. A total of 17 alleles with an average of 3.4 alleles per locus were detected. The polymorphic information content (PIC) among genotypes varied from 0.076 (TC26 285) to 0.673 (AG14). Pairwise coefficients of genetic similarity between all genotypes ranged from 0.24 to 1.00. Unweighted pair-group method arithmetic average (UPGMA) analysis allocated the genotypes and their parents in 4 major clusters. Cluster I, consists of 6 genotypes and Siam Madu with 89% genetic similarity Cluster II consist of 14 genotypes with 91% genetic similarity. Cluster III consist of F17 and cluster IV consist of Satsuma Mandarin, with 58,6% and 24% genetic similarity, respectively. The genetic distance between all somatic hybrid genotypes with the parental Satsuma Mandarin is 0.60, except for F17 the genetic similarity is 0.34. Keywords: Genetic, somatic hybrid, SSR markers, Siam Madu, Satsuma Mandarin.
Authors and Affiliations
C. Martasari, S. Ashari, L. Sulistyowati, A. Husni
Keywords
Related Articles
- EP ID EP380351
- DOI -
- Views 155
- Downloads 0
Isolation of Escherichia coli and Salmonella sppfrom Japanese quail (Coturnixcoturnix japonica) raw eggs from selected farms in Sokoto metropolis, Nigeria
In Nigeria, demand for quail eggs and meat in recent years has been unprecedented as most of the consumers believed that the eggs have medicinal value with most of them taking the eggs raw. These claims have public healt...
Effects ofMoringaLeaf and Fruit Powders and a Commercial Herbal Preparation on the Organ Size, Ileal Digestibility and Intestinal Microflora of Broiler Chickens
A floor pen experiment was carried out to investigate the effects of Moringaoleifera and a commercial herbal preparationZigbir on the organ size, ileal digestibility and intestinal microflora of broiler chicken. Total o...
Evaluation of Energy Utilization and Haematological Indices of West African Dwarf Goats Fed Varying Levels of Yam Peels and Cowpea Husk.
Twenty four West African dwarf goats balanced for weight (7.00+0.25kg) were fed varying levels of yam peels and cowpea husk to evaluate their energy utilization and haematological indices. Goats were allotted to three di...
Surgical excision of leomyosarcoma in a Pomeranian dog
Abstract:Leiomyosarcoma of somatic soft tissues, like other soft tissue sarcomas, often present as an enlarging, painless mass. Leiomyosarcoma is an aggressive soft tissue sarcoma derived from smooth muscle cells typical...
Controversies of Origin of Domestic Dog - II - References of Fossil Dogs
Abstract:The dog family or Canidae is a group of carnivore mammals in which the dog, Canis familiaris, is the only member to have been domesticated, although the red fox and the raccoon from time to time have been bred f...