Genetic Identification of Siam Madu and Satsuma Mandarin Somatic Hybrid Genotypes Using SSR Markers
Journal Title: Scholars Journal of Agriculture and Veterinary Sciences - Year 2014, Vol 1, Issue 2
Abstract
Abstract: Simple sequence repeat (SSR) analysis was used to identify the genetic status 23 citrus somatic hybrid genotypes from Siam Madu +Satsuma Mandarin and their similarity to the parental. Five SSR primer pairs could amplify polymorphic SSRs from all of these genotypes. A total of 17 alleles with an average of 3.4 alleles per locus were detected. The polymorphic information content (PIC) among genotypes varied from 0.076 (TC26 285) to 0.673 (AG14). Pairwise coefficients of genetic similarity between all genotypes ranged from 0.24 to 1.00. Unweighted pair-group method arithmetic average (UPGMA) analysis allocated the genotypes and their parents in 4 major clusters. Cluster I, consists of 6 genotypes and Siam Madu with 89% genetic similarity Cluster II consist of 14 genotypes with 91% genetic similarity. Cluster III consist of F17 and cluster IV consist of Satsuma Mandarin, with 58,6% and 24% genetic similarity, respectively. The genetic distance between all somatic hybrid genotypes with the parental Satsuma Mandarin is 0.60, except for F17 the genetic similarity is 0.34. Keywords: Genetic, somatic hybrid, SSR markers, Siam Madu, Satsuma Mandarin.
Authors and Affiliations
C. Martasari, S. Ashari, L. Sulistyowati, A. Husni
Keywords
Related Articles
- EP ID EP380351
- DOI -
- Views 150
- Downloads 0
Confirmation of Endocrine Cells in the Pancreas of Iguana iguana with Immunohistochemistry
Pancreatic endocrine cells of Green iguana (Iguana iguana) could not be distinguished clearly with routine histology staining (hematoxylin and eosin stain). Immunohistochemical method was carried out to detect the presen...
Assessment of Integrated Soil Fertility Management System on the Growth and Yield of Abelmoschus esculentus in a Tropical Alfisol
The combined effect of Poultry manure, Pig slurry and NPK fertilizer as integrated nutrient on soil fertility and on the growth and yield of Abelmoschus esculentus (Okra) were examined on a tropical Alfisol, under field...
Farm Management of Foot-Rot in Sheep
Abstract: Foot-rot is a commonly occurring disease of sheep that is cause by Dichelobacter nodosus. Twenty animals were suffering from foot-rot. After isolation of the diseased animals provide the antibiotic and anti-inf...
Genetic Diversity Studies of Bacteria Associated with Brycinus longipinnis from Eggua on Yewa River using Random Amplified Polymorphic DNA (RAPD) Markers
Twenty samples of Brycinus longipinnis were collected from Eggua landing site on Yewa river Ogun state, which lies on latitude 7o03ˈN and longitude 2o55ˈE. The water quality parameters (Temperature, Dissolve Oxygen and p...
Genetic Analysis of Plant Potassium Accumulation under Potassium Deficiency Stress at Filling Stage in Maize (Zea Mays L.)
Six generations, including P1，P2，F1，B1，B2 and F2, were constructed by 90-21-23 (tolerance to K deficiency) and D937 (sensitive to K deficiency) in maize (Zea Mays L.). The genetic analysis of plant potassium Accumula...