Genetic polymorphisms of GSTM1 and GSTT1 and susceptibility to primary DNA damage in steel industry workers
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 4
Abstract
The present study aims to investigate the possible association of detoxifying gene polymorphisms with DNA damage in steel industry workers. 150 steel industry workers and 146 control subjects with no history of occupational exposure to steel dust or any other chemicals were recruited for the study. DNA damage was evaluated using simple and reliable alkaline comet assay in peripheral blood lymphocytes of male steel industry workers. Analysis of GSTM1 and GSTT1 gene polymorphisms was done by multiplex PCR method. The results showed an increase in the frequency of DNA damage in peripheral blood lymphocytes of the steel industry workers compared to the control subjects. The statistical analysis of the results showed that significant DNA damage was observed in both genotypes of GSTM1 and GSTT1 but the frequency was found to be lower in steel industry workers with wild GSTM1 and GSTT1 genes than in those with null genotypes. The findings in this study presented evidence for the DNA damage highlighting an increased genotoxic risk as a result of exposure to dust in steel industry workers. The study also presented evidence for an association of both null genotypes of GSTM1 and GSTT1polymorphisms with DNA damage indicating the influence of GSTM1 and GSTT1 polymorphisms on this biomarker.
Authors and Affiliations
U. Indira Priyadarshini, Vanitha B. , Ch. Prashanth, Sridevi T. , Priyanka P. , P. Pranay Krishna, Latheef SAA, P. P Reddy
Keywords
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