Genetic structure in anzali wetland’s pike (Esox lucius) using microsatellite molecular method
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 6, Issue 3
Abstract
In this research, Pike (Esox lusius) one of the most valuable commercial marine species has been evaluated for genetic structure in Anzali wetland using microsatellite markers. 60 specimens of adult pikes were sampled from two spawning seasons, winter and spring in Anzali wetland. Five pairs of microsatellites, tested on the genomic deoxyribonucleic acid (DNA). All loci of microsatellite produced polymorphic bands as polymorphic loci were used to analyze the genetic variation of the pike. Analyses revealed that average of alleles per locus were 10.8 (range 9 to 13 alleles). All sampled seasons contained private alleles. The average observed and expected heterozygosity was 0.914 and 0.885, respectively. The inbreeding coefficient values of five microsatellite loci were negative. With the exception of a locus in spring, all loci significantly deviated from H-W equilibrium (P<0.01). Based on AMOVA, RST and FST values were significant between seasons (P<0.01). The genetic distance between populations was 0.442, which indicates that the genetic difference among the studied populations is significant. These results support the existence of different genetic populations in spawning seasons in this area.
Authors and Affiliations
Mohammad Hadi Samiei, Mehrnoush Norouzi, Amin Ravaei, Mohammad Behrooz, Ali Nazemi
Evaluation of human induced threats on berberis populations across cultural landscape of karakorum mountain ranges
Growing human and livestock population have negatively influenced Berberis populations across traditional landscape of Karakoram Mountain Ranges. Current study evaluates human induced pressures and threats on Berberis....
An investigation on the mechanism of land subsidence in the northwest of mashhad city, NE Iran
Mashhad city is located in Mashhad Plain in the northeast of Iran. Mashhad plain is one of the most important plain in Khorasan Razavi province. In the last decades, rapid population growth coupled with agricultural ex...
Relationships among morpho-phenological traits using principal components analysis in safflower
Safflower (Carthamus tinctorius L.) is an oilseed crop which is adapted to semi-arid region of the world. The objective of this study was to use statistical method of principal components analysis to evaluate the relati...
Investigation in the intergenic transcribed sequences to determination of genetic variation for Iranian species of salvia
The nuclear ribosomal DNA internal transcribed spacer (ITS) has been established as a suitable genomic region to clarify genomic and phylogenetic relationships of plant species. This study aimed to evaluate the performan...
Valuation of solid benefits of a homestead forestry system in the Indus basin, Southern Punjab, Pakistan.
To evaluate the benefits of homestead forestry system to the farmers, a study was conducted in southern Punjab (Pakistan) with the hypothesis that “Homestead Forestry system has tremendous potential to enhance the econom...