Genetic transformation of Vanda lilacina Teijsm. & Binnend. with a chitinase gene.
Journal Title: Advances in Agriculture & Botanics - International Journal of the Bioflux Society - Year 2010, Vol 2, Issue 1
Abstract
This research was aimed to improve plantlet regeneration and to establish the optimal conditions for transformation of Vanda lilacina Teijsm. and Binnend. using Agrobacterium tumefaciens strain LBA4404 (pCAMBIA 1305.1) harboring a chitinase gene as a fungal resistant one. It was found that seeds successfully germinated, and developed into protocorms and plantlets on solid New Dogashima (ND) medium supplemented with 1 % (w/v) potato juice. Protocorm proliferation was successful when culturing the protocorms on solid ND medium supplemented with 3 mg/l BA. For successful genetic modification, the protocorms were cocultivated with A. tumefaciens for 45 min. Bacterial elimination and selection of putative transformants were implemented using 250 mg/l cefotaxime and 10 mg/l hygromycin, respectively. GUS assay revealed 80% of the protocorms expressing gus gene activity. Availability of 35S and NOS detected by PCR analysis confirmed gene integration.
Authors and Affiliations
Witaya Pimda, Sumontip Bunnag
Keywords
Related Articles
- EP ID EP124088
- DOI -
- Views 122
- Downloads 0
Salt tolerance of genetic modified potato (Solanum tuberosum) cv. Agria by expression of a bacterial mtlD gene
Water and salinity stresses are the major reasons to yield decreasing in the world. Potato is the world’s main tuber crops of the Solanaceae family which is one of the most economically important annual vegetable c...
Cleome gynandra L. (C4 plant) shows higher tolerance of salt stress than its C[sub]3[/sub] close relative, C. viscosa L.
The effect of salt stress on some physiological and anatomical parameters in two species of Cleome, C. gynandra (C[sub]4[/sub] plant) and C. viscosa (C[sub]3[/sub] plant) was investigated. Seedlings were grown in potted...
The development, the present situation and the prospects of Greek agriculture.
There is a general consensus that the future of the European agricultural sector faces great insecurity. However, while agricultural exodus continues to constitute, in many Southern European Union regions, one of the mos...
Different tillage and maize grass intercropping on root systems, growth and yield of rainfed maize (Zea mays L.)
The study was conducted in a year period in rainfed area of Lampung province Indonesia. Objective of this study was observe the effect of tillage and maize grass intercropping in the rainy season to succeeding dry...
Genetic association and path coefficient analysis in mung bean Vigna radiata (L.) Wilcze
Genetic variability and character association in 23 genotypes of mung bean for different quantitative characters were studied in kharif (summer or monsoon crop) 2007. The analysis of variance revealed highly significan...