Genetic transformation of Vanda lilacina Teijsm. & Binnend. with a chitinase gene.
Journal Title: Advances in Agriculture & Botanics - International Journal of the Bioflux Society - Year 2010, Vol 2, Issue 1
Abstract
This research was aimed to improve plantlet regeneration and to establish the optimal conditions for transformation of Vanda lilacina Teijsm. and Binnend. using Agrobacterium tumefaciens strain LBA4404 (pCAMBIA 1305.1) harboring a chitinase gene as a fungal resistant one. It was found that seeds successfully germinated, and developed into protocorms and plantlets on solid New Dogashima (ND) medium supplemented with 1 % (w/v) potato juice. Protocorm proliferation was successful when culturing the protocorms on solid ND medium supplemented with 3 mg/l BA. For successful genetic modification, the protocorms were cocultivated with A. tumefaciens for 45 min. Bacterial elimination and selection of putative transformants were implemented using 250 mg/l cefotaxime and 10 mg/l hygromycin, respectively. GUS assay revealed 80% of the protocorms expressing gus gene activity. Availability of 35S and NOS detected by PCR analysis confirmed gene integration.
Authors and Affiliations
Witaya Pimda, Sumontip Bunnag
Keywords
Related Articles
- EP ID EP124088
- DOI -
- Views 119
- Downloads 0
The analysis of the effects of rurbanization on rural communities in the North-East Development of Romania.
This paper highlights the effects of the rurbanization phenomenon on rural communities in the North-East Development Region of Romania The paper summarizes the results of field research conducted in the Jijia – Bahlui De...
Macrofungi from „Tinovul de la Româneşti” peat bog (Dornelor Depression, Romania).
„Tinovul de la Româneşti” is a forested peat bog, with an area of 20 ha, located in Dornelor Depression (Eastern Carpathians) within the limits of Coşna commune, Suceava County, Romania. The mycological observations carr...
Effect of exogenous proline on protein pattern changes in Citrus sinensis (L.) Osbeck under in vitro salt stress
Effect of exogenous proline treatment on growth and protein pattern changes of plantlets of Citrus sinensis (L.) Osbeck cv. Local orange under NaCl stress was investigated. Under salt stress conditions, the height and le...
The influence of climatic conditions on the main vine diseases in terms of chemicals application
This paper presents research results on the influence of climatic conditions on the occurrence and manifestation of main vine diseases. In the Blaj wine center the main grapes diseasesare blight (Plasmopara viticola), mi...
Chemical and bio-chemical studies of new varieties of safflower (Carthamus tinctorius L.) PBNS-12 and PBNS-40 seeds
Seeds of new safflower (Carthamus tinctorius L.) variety (PBNS-12 and PBNS-40) were analyzed for contents of moisture, crude fiber, lipids, proteins, carbohydrates, minerals, energy, toxic compounds (such as cyanogenic g...