Genetic variability of broodstocks of restocking programs in Brazil
Journal Title: Revista MVZ Cordoba - Year 2015, Vol 20, Issue 3
Abstract
Objective. The aim of this study was evaluate the genetic diversity of the following broodstocks: piapara (Leporinus elongatus), dourado (Salminus brasiliensis), jundiá (Rhamdia quelen) and cachara (Pseudoplatystoma fasciatum) already useful for restocking programs in the Paranapanema, Iguaçu and Paraná Brazilian Rivers. Materials and methods. Samples from the caudal fin of 122 fish were analyzed. DNA was extracted by NaCl protocol. PCR products were separated by a horizontal agarose gel electrophoresis. The fragments were visualized by staining with ethidium bromide. Results. The amplification of 25 primers generated different fragments in studied species that allowed characterizing 440 fragments of 100-2900 bp. High percentage of polymorphic fragments (66.67 to 86.29), Shannon index (0.365 to 0.486) and genetic diversity of Nei (0.248 to 0.331) were detected. Conclusions. The level of genetic variability in the broodstocks was adequate for allowing their use in restocking programs in the studied Rivers. However, periodical monitoring studies of genetic variability in these stocks, the mating system, reproductive system and general management must be made to guarantee the preservation of wild populations.
Authors and Affiliations
Nelson Lopera-Barrero, Ph. D *| Universidade Estadual de Londrina, Departamento de Zootecnia, Programa de Pós-Graduação em Ciência Animal, Rod. Celso Garcia Cid Pr 445 hm 380 Campus Universitário, Londrina, Brasil.Correspondence: nmlopera@uel.br, Ed Lima, Zoot| Universidade Estadual de Londrina, Departamento de Zootecnia, Programa de Pós-Graduação em Ciência Animal, Rod. Celso Garcia Cid Pr 445 hm 380 Campus Universitário, Londrina, Brasil., Luiz Filho, Ph. D| Universidade Estadual de Maringá, Departamento de Zootecnia, Programa de Pós-Graduação em Zootecnia, Avenida Colombo 5790 Jardim Universitário, Maringá, Brasil., Elenice Goes, M. Sc| Universidade Estadual de Maringá, Departamento de Zootecnia, Programa de Pós-Graduação em Zootecnia, Avenida Colombo 5790 Jardim Universitário, Maringá, Brasil., Pedro Castro, Zoot| Universidade Estadual de Maringá, Departamento de Zootecnia, Programa de Pós-Graduação em Zootecnia, Avenida Colombo 5790 Jardim Universitário, Maringá, Brasil., Aline Zardin, M. Sc| Universidade Estadual de Maringá, Departamento de Zootecnia, Programa de Pós-Graduação em Zootecnia, Avenida Colombo 5790 Jardim Universitário, Maringá, Brasil., Angela Poveda-Parra, Ph. D| Universidade Estadual de Londrina, Departamento de Zootecnia, Programa de Pós-Graduação em Ciência Animal, Rod. Celso Garcia Cid Pr 445 hm 380 Campus Universitário, Londrina, Brasil., Ricardo Ribeiro, Ph. D| Universidade Estadual de Maringá, Departamento de Zootecnia, Programa de Pós-Graduação em Zootecnia, Avenida Colombo 5790 Jardim Universitário, Maringá, Brasil.
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