Genetic Variation among Dwarf Gourami (Trichogaster lalia, Osphronemidae) populations using Random Amplified Polymorphic DNA(RAPD) markers
Journal Title: Journal of Research in Biology - Year 2015, Vol 5, Issue 3
Abstract
Random amplified polymorphic DNA (RAPD) was applied to generate species-specific diagnostic fragment patterns for the molecular identification of the ornamental aquarium fish species Trichogaster lalia, more commonly known as dwarf gourami. The species were collected from various geographically distant locations of Assam. After initial screening, four primers having a length of 10 arbitary nucleotide sequence were used which generated the RAPD profile for Trichogaster species. The primers produced 39 bands in total. In the experiment 22 polymorphic bands and 7 monomorphic bands were produced. The genetic distance of an individual ranged from 0.03 to 0.38. The average genetic distance among the individuals showed that more than 0.03 species are genetically more similar
Authors and Affiliations
Merina Das, Goswami MM
Keywords
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