Genetical diversity analysis of Iranian Fusarium oxysporum f.sp. melonis by PCR - RAPD marker
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 23
Abstract
Fusariums wilt of melon, Fusarium oxysporum f.sp. melonis is one of the most important diseases, causing tremendous losses in melon growing areas in Iran. Searching for resistant sources needs detail knowledge of the pathogen. Thus, genetical diversity of the pathogen were assessed on various isolates through out of important melon's growing areas, using differential standard hosts and molecular marker PCR - RAPD. The results indicated that, eighty percent of the isolates are pathogenic to the melon crops. Forma specials proving tests on seedlings of melon, cucumber, watermelon and pea showed typical symptoms of the disease on melon plants, confirming the only forma specials of melon. For the race identification tests, differential hosts were used, out of which, the existence of race one was approved. PCR - RAPD profile showed a polymorphism among populations of the pathogen and cluster analysis divided them into seven genotypic groups accordingly.
Authors and Affiliations
Mahdi Nasre Esfahani, Noushin Shafagh, Mahrokh Falahati Rastegar and Raziyeh Malekiyan
Keywords
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