Genome specificity of primers to puroindoline genes
Journal Title: Фактори експериментальної еволюції організмів - Year 2018, Vol 22, Issue
Abstract
Aim. Genome specificity of primers for amplification of complete coding sequences of puroindoline genes was studied. Methods. PCR with gene-specific primers was performed for amplification of puroindoline genes of wheat and related species. Results. The primer pair designated PinaDH is not gene-specific as it yields two products of amplification of the genes Pina-1 and Gsp-1. This primers pair is not specific for the Pina-1 gene of the genome V of D. villosum. The primer pairs designated PinaCM and Pinb are gene-specific as they produce one amplification product of respective length. We have demonstrated that the PinaCM primer pair is also specific for the genomes D, E and V, and the Pinb primer pair is not specific for the genome V. Conclusions. Gene and genomic specificity of primers for puroindoline genes was refined. Primers suitable for further direct sequencing of coding parts of puroindoline genes of wheat and related species were chosen. Keywords: puroindoline, Triticum aestivum, Aegilops, Dasypyrum, genome specificity.
Authors and Affiliations
О. І. Созінова, Н. О. Козуб, І. О. Созінов, Я. Б. Блюм
Стійкість мікроорганізмів чорноземного ґрунту до розчинних сполук міді
Мета. Визначення стійкості мікроорганізмів чорноземного ґрунту України до дії токсичної міді(II). Методи. Вміст мідь-резистентних мікроорганізмів у чорноземі визначали підрахунком колоній на твердому поживному середовищі...
The analisis of human MGMT gene orthologous in protests
Aim. The intron sequences of orthologous О6-methylguanin-DNA methyltransferase (MGMT) genes in Protists on the early stages of their formation in eukaryotic organisms have been analysed. Methods. Homologous regions have...
Productivity of winter wheat plants under drought
Aim. The goal of research was to study the effects of soil drought on the productivity of winter wheat plants (Triticum aestivum L.). Methods. Wheat plants of the cultivars of Zolotocolosa and Astarta were grown under op...
Applying the nanobiotechnological methods for optimize the in vitro cultivation technology for cows oocytes
Aim. Investigated effect nanomaterial of highly dispersed ultra fine silica (UFS) by carbohydrate – sucrose (UFS/sucrose) on the effectiveness of meiotic maturation cows oocytes in vitro. Methods. The fresh and frozen –...
Puffing activity in Drosophila melanogaster Meig. political chromosomes after exposure to microwave radiation
Aim. The aim of the work was to study the effect of microwave radiation of varying intensity on the polytene chromosomes puffing activity in larvae salivary glands of Drosophila melanogaster. Methods. The wild type outbr...