Genotyping identification and sequence analysis of ABO ambiguous blood group in 20 cases
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 1
Abstract
Objective To study the genotypes of ABO ambiguous blood group samples(n=20) and identify their molecular biological characteristics. Methods The serological phenotype of the samples was analyzed by serological techniques. Seven exons of ABO gene were amplified by polymerase chain reaction (PCR) and the PCR products were directly sequenced; the genotypes and sequences of ABO subtypes were analyzed. Results The serological phenotypes of 20 samples presenting ABO ambiguous blood group were as follows: weak A antigen (n=5), weak A antigen combined with anti-A1 antibody (n=5), normal A antigen combined with anti-A1 antibody (n=2), weak B antigen (n=8). The genotypes of them were as follows: Ax02/O01 (n=3), Ael07/O01 (n=2), B313/O01 (n=2), A204/O02 (n=1), A220/O01 (n=1), Ael07/O02 (n=1), Ael02/O01 (n=1), Ael02/O02 (n=1), Ax03/O01 (n=1), Ax03/O02 (n=1), B313/O02 (n=1), B302/O01 (n=1), B302/O02 (n=1), Bw19/O02 (n=1), A102/B313 (n=1) and A101/Bw37 (n=1). Conclusion ABO genotyping technology can accurately identify the ambiguous blood group of samples, provide definite genetic information of blood group and ensure the safety of clinical transfusion.
Authors and Affiliations
Qiuyan LIN, Jinping ZHANG, Zhenyu HUANG, Qinghua HUANG, Liping FAN, Danhui FU, Haobo HUANG
Keywords
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- EP ID EP738453
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.01.002
- Views 59
- Downloads 0
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