Genotyping of Clinical Isolates of Salmonella enterica serovar Typhimurium from Medical Centers of Kerman Province Using ERIC-PCR Method
Journal Title: مجله علمی- پژوهشی دانشگاه علوم پزشکی قم - Year 2018, Vol 12, Issue 1
Abstract
Background and Objectives: Salmonella enterica serovar Typhimurium is one of the most common Salmonella serotypes in gastroenteritis cases. ERIC-PCR method is used for genotyping studies and examinations in order to apply appropriate preventive and controlling conditions. This research was carried out with the objective of investigating the genotyping of Salmonella typhimurium strains in the treatment centers of Kerman province. Methods: In this descriptive study, different strains of Salmonella Typhimurium, were isolated from different treatment centers. All strains were examined using standard methods of microbiology, biochemistry, and molecular biology. Genetic relationship between the strains was evaluated by ERIC-PCR method. Results: In this study, Among 891 stool and blood samples of patients with diarrhea, 48 strains of Salmonella typhimurium, were isolated. The isolates were classified in terms of genotyping into 15 different groups using ERIC-PCR method, which the highest number of the strains was in the 14th group (25%, 12 strains). Conclusion: The results of the present study showed high discrimination power of ERIC-PCR method for molecular typing and genetic diversity of Salmonella typhimurium strains isolated from human samples. Therefore, this method can be used for epidemiological studies in order to investigate the source of contamination, genetic diversity and its relationship with geographical distribution and drug resistance of the isolated strains.
Authors and Affiliations
Abolfazl Abolfazl, Shahram Shahram
فراوانی استفاده و رضایتمندی دندانپزشکان شهر قم از سیستمهای مختلف ایمپلنتهای دندانی
زمینه و هدف: با توجه به روند روزافزون استفاده از ایمپلنت، بسیاری از دندانپزشکان خواهان استفاده از ایمپلنتهایی با حداکثر کیفیت و سهولت کار هستند؛ بنابراین مطالعه حاضر با هدف بررسی فراوانی استفاده و رضایتمندی دندانپزشکان...
Evaluation of Anticandidal Effects of Allium saralicum
Background and Objectives: Candida species is one of the main fungal pathogens in immunodeficient, organ transplantation, and cancer patients. Resistance to antifungal therapy, is observed in candida species, such as Can...
تأثیر عصاره هیدروالکلی زیرهسبز بر اضطراب در موشهای صحرایی نر گنادکتومیشده
زمینه و هدف: اضطراب یکی از اختلالات روانی رایج در بین جمعیتهای انسانی و یک احساس ناراحتکننده است که خطری نامعلوم و مبهم را تداعی میکند. در این مطالعه، اثر عصاره هیدروالکلی زیرهسبز بر اضطراب در موش صحرایی نر گنادکتومی...
The Effectiveness of Neurocognitive Rehabilitation On the Math Performance and Working Memory of Students with Dyscalculia
Background and Objectives: Cognitive functions are one of the most important effective factors in students with dyscalculia. The present research aims to investigate the effectiveness of neurocognitive rehabilitation pro...
میزان علاقهمندی به پژوهش در دانشجویان دانشگاه علوم پزشکی قم، سال 1395
زمینه و هدف: پژوهش از عوامل مهم در توسعه و پیشرفت جوامع بوده و علاقهمندی به فعالیتهای پژوهشی نیز بر گرایش به انجام فعالیت در این زمینه مؤثر است. مطالعه حاضر با هدف تعیین میزان علاقهمندی به پژوهش در دانشجویان دانشگاه ع...