GEOGRAPHICAL IMPACTS OF PODOPHYLLOTOXIN (ANTI-CANCER DRUG AGENT) FOUND IN ROOTS OF PODOPHYLLUM HEXANDRUM & YIELD ENHANCEMENT USING PRECURSORS AND ELICITORS: LC-MS/MS METHOD
Journal Title: International Journal of Medicine and Pharmaceutical Sciences (IJMPS) - Year 2017, Vol 7, Issue 3
Abstract
Podophyllum hexandrum is a well-known medicinal plant, which demonstrates numerous therapeutic effects due to the presence of Podophyllotoxin, especially in Oncology. In this study, after development and validation of an RP-HPLC-MS method, levels of Podophyllotoxin in root samples of this plant collected from north of the Himalayas were measured, and environmental factors affecting its content were investigated. Five groups of plant populations from different spots of northern Himalayas were collected, and concentration levels of Podophyllotoxin were measured applying a validated High Performance Liquid Chromatography- Diode Array Detector/Mass Spectrometry (HPLC-DAD/MS) method. The impact of geographical parameters encompassing altitude and an average temperature of Podophyllotoxin contents was assessed. The validated method was selective, with good resolution, excellent linearity (r2 > 0.9997), high accuracy, sensitivity and precision. The results illustrated that there was a direct correlation between altitude with the content of Podophyllotoxin in plant, which means that more the altitude, the more the content of Podophyllotoxin. In an opposite manner, levels of the Podophyllotoxin reversely correlated with the average temperature, in a way that, decreasing this variable resulted in raising the amount of the Podophyllotoxin. Treatments of cultured cells with fungal electors have been shown to induce the phenylpropanoid/flavonoid biosynthetic pathways and electors methyl jasmonate at 15 µM after 4 days resulted in higher HPLC content (8.606 %) and of isosafrole at 10 µM after 8 days shows 5.244%.
Authors and Affiliations
D. K SHARMA, ANIL KUMAR, ASHIMA SHARMA
Keywords
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- EP ID EP226005
- DOI 10.24247/ijmpsjun20173
- Views 168
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