GFAP EXPRESSION IN NEUROGLIAL TUMOURS- IMMUNOHISTOCHEMICAL CONFIRMATION FOR DIAGNOSIS AND GRADING
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 46
Abstract
BACKGROUND GFAP is a reliable marker for differentiating glial tumours from non-glial tumours. In addition, the percentage and intensity of staining helps in grading of glial tumours. The aim of this study was to determine the distribution of Glial Fibrillary Acidic Protein (GFAP) in neuroglial tumours and its correlation with histologic grading. MATERIALS AND METHODS Representative formalin-fixed paraffin-embedded blocks were selected for 51 cases of CNS tumours. 3 micrometer thick sectionswere cut from each block and sections were taken on poly-L-lysine coated slides. Immunostaining was done by Streptavidin-biotin immunoperoxidase technique (LSAB) using antibody to Glial Fibrillary Acidic Protein. Descriptive statistics was used to show characteristics of collected data. On application of Kruskal-Wallis test, a significant inverse correlation was found between grade of tumour and total average GFAP staining score with statistically significant p < 0.002. Study Design- Cross-Sectional Study. RESULTS In the current study, all glial tumours were stained positive for GFAP. The average score of Grade 1 tumours was 6.7, Grade 2 tumours was 6.6, Grade 3 tumours was 5.75 and that of Grade 4 tumours was 5.2. CONCLUSION GFAP is a reliable marker to differentiate between glial and non-glial tumours, and a high degree of malignancy is associated with reduced cellular differentiation as shown by reduced GFAP staining. Therefore, GFAP IHC is complimentary to the histological diagnosis and grading of brain tumours.
Authors and Affiliations
Amit V. Varma, Garima Gupta, Jagdish Gupta, Sapan Gupta
Keywords
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- EP ID EP548084
- DOI 10.14260/jemds/2018/1120
- Views 61
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