Glutathione: methods of sample preparation for chromatography and capillary electrophoresis
Journal Title: CHEMIK nauka-technika-rynek - Year 2012, Vol 66, Issue 9
Abstract
Reduced form of glutathione is one of the most important antioxidants, which is found in human body. Deficiency of this compound can often lead to serious disorders. It plays a central role in immunity modulation, detoxification capacities and regulation of activity of another peptides and enzymes. In every live organism it acts as a sensitive rate of susceptibility to oxidative stress and its concentration in each organ and cell shows the level of their damage. Determination of glutathione using chromatographic methods has been a complement and many a time is also an alternative to different widely used methods, such as: enzymatic, spectrophotometric, spectrofluorometric, electromigrative, chemiluminescence, nuclear magnetic resonance. The most important steps in the sample preparation process for analysis are: amino thiols protection from high temperature and oxidation caused by substances from matrix, precipitation, reduction of disulfides and also derivatization (except for in vivo assays and those based on electrochemical and tandem mass spectrometry). Several methods have been presented in order to identify and quantify glutathione forms in human biological samples such as liquid chromatography with electrochemical, UV, fluorometric, mass spectrometric detection capillary electrophoresis and gas chromatography with mass spectrometry. This review presents different techniques for glutathione routine analysis in hospitals and also useful in clinical researches with large groups of patients.
Authors and Affiliations
Ewelina Błońska-Sikora, Jerzy Oszczudłowski, Zygfryd Witkiewicz, Dariusz Wideł
Keywords
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