Glutathione Reductase of Vacuole. Comparison of Glutathione Reductase Activity of Vacuole and Tissue Extract of Red Beet Root (Beta vulgaris L.)
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2016, Vol 12, Issue 1
Abstract
Glutathione reductase (GR, EC 1.8.1.7) is the enzyme that reduces oxidized glutathione (GSSG) and thus regulates the redox state of glutathione (GSH/GSSG). GR has been studied in most plants. This enzyme has been identified in chloroplasts and cytosol, so these cellular compartments are considered to be the main place of the enzyme localization. In the same time, just a little is known about GR vacuoles. There are no conclusive evidences to prove the presence or absence of this enzyme in the vacuoles. GR activity was found in the vacuoles of red beet root cells ([i]Beta vulgaris[/i] L.). The level of activity, the optimum pH and isoenzyme composition of GR were compared in the vacuoles and tissue extract of beet root. Vacuolar GR activity was quite high, it was 1.5-2 times higher than the activity of the tissue extract. Enzyme pH optimum of all the objects were identical. pH-optimum depend on the pyridine nucleotide nature: pH 7.0-8.0 was an optimal range with NADPH; pH 5.0 – with NADH. GR activity of the vacuoles and tissue extracts decreased in the presence of a noncompetitive inhibitor 1-chloro-2.4-dinitrobenzene (CDNB), indicating the specificity of this enzymatic reaction. Two bands with glutathione reductase activity have been identified in the vacuoles and tissue extracts using zymography method to determine the enzymatic activity in PAAG after electrophoresis of proteins. Belonging to the GR isoforms of these bands was confirmed by enzyme immunoassay (Western blotting). The electric mobility of isoforms of the study objects did not differ significantly. It is concluded that the biochemical characteristics of vacuolar glutathione reductase were substantially identical to the biochemical characteristics of other localization GR.
Authors and Affiliations
E. Pradedova, O. Nimaeva, R. Salyaev
Keywords
Related Articles
- EP ID EP101378
- DOI -
- Views 72
- Downloads 0
Lead (Pb) heavy metal impacts in the green Ulva lactuca (Chlorophyceae) marine algae
Toxicity of different lead (Pb) (0, 2, 4 and 8 mg/L) concentrations in the green [i]Ulva lactuca[/i] (Chlorophyta) marine algae at physiological level has been investigated 48 h after Pb treatment under laboratory condit...
Influence of N-phenyl-2-naphthylamine on the Activity of Adenylate Cyclase Signaling System and the Virulence of Clavibacter michiganensis subsp. sepedonicus
The effect of N-phenyl-2-naphthylamine was obtained from exudates of pea root on growth, virulence and signaling-specific of potato phytopathogen [i]Clavibacter michiganensis[/i] subsp. [i]sepedonicus[/i]. It is shown th...
Alterations of protein and DNA profiles of Zea mays L. under UV- B radiation
UV radiation is an important stress factor for plants which may result in damage to genetic system and cell membranes and several metabolic processes. UV-B has greater damaging effects on plants because the cell macromol...
Does Glycine Betaine and Salicylic Acid Ameliorate the Negative Effect of Drought on Wheat by Regulating Osmotic Adjustment through Solutes Accumulation?
A pot experiment was conducted to evaluate the beneficial effect of foliar application of glycine betaine (10mM), grain presoaking in salicylic acid (0.05 M) and their interaction on drought tolerance of two w...
Flavonoids in Adaptation of Begonia grandis Dryander subsp. grandis Introduced in West Siberia (Novosibirsk)
The dynamics of flavonoids (flavones and flavonols) in leaves of [i]Begonia grandis[/i] Dryander [i]subsp. grandis[/i] plants introduced in Western Siberia (Central Siberian Botanical Garden, Siberian Branch of the Russi...