GLYCATION INHIBITORS AND PROBIOTICS CAN AMELIORATE THE CHANGES CAUSED BY HIGH FRUCTOSE FEED
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 7
Abstract
Objective: To evaluate the use of protein glycation inhibitors and probiotics to ameliorate secondary complications in diabetes and to improve gut microbiota respectively in high fructose fed Wistar rat.Methods: The study was conducted on male Wistar rats for 7 d. Blood glucose levels in oral glucose tolerance test (OGTT) were measured using glucometer, serum parameters were analyzed using commercial kits, antioxidant status was evaluated by measuring superoxide dismutase (SOD) and catalase (CAT) levels, total reactive oxygen species were estimated using a fluorescent 2’, 7’-dichlorofluorescin diacetate (DCF-DA) dye, and tissue fluorescence of liver, kidney and intestine were measured using a spectrofluorimeter.Results: OGTT pattern shows significant increase in blood glucose of fructose fed rats i.e. 154 mg/dl while, in aminoguanidine (AMG) treated and gut microbiota modulated animals it is 137 and 119 mg/dl resp. after 30 min on glucose administration. Marked reduction was found in SOD 6.37 and 11.25 U/mg of protein and catalase 186 and 65.5 U/mg of protein in liver and kidney of fructose fed animals when compared to fructose+AMG and fructose+EUGI. There is 5-6 fold significant increase in general and specific tissue fluorescence of liver and kidney, and 2.2 fold increase in liver reactive oxygen species was observed in fructose fed group as compare to control animals. Significantly higher glycation was found in intestine of fructose fed animals (general fluorescence 2.1 and specific fluorescence 3.1 AU/mg), more than that of diabetic control rats (general fluorescence 0.9 and specific fluorescence 1.6 AU/mg), represented an evidence for adverse impact of excess fructose on healthy gut.Conclusion: The use of protein glycation inhibitor and use of pre and probiotics significantly improved the serum parameters and would prevent progression to secondary complications.
Authors and Affiliations
Rahul S. Patil, Ashwini D. Jagdale, Megha L. Nalawade, Laxman N. Bavkar, Akalpita U. Arvindekar
Keywords
Related Articles
- EP ID EP566213
- DOI 10.22159/ijpps.2018v10i7.26870
- Views 47
- Downloads 0
DISSOLUTION BEHAVIOR OF CARBAMAZEPINE SUSPENSIONS USING THE USP DISSOLUTION APPARATUS 2 AND THE FLOW-THROUGH CELL METHOD WITH SIMULATED GI FLUIDS
Objective: To characterize the dissolution behaviour of carbamazepine generic suspensions using the USP Dissolution Apparatus 2 and the flow-through cell method with simulated gastrointestinal fluids as dissolution media...
CARDIOVASCULAR DISEASE PREVALENCE AND DRUG UTILIZATION PATTERNS AT A TERTIARY CARE HOSPITAL IN NORTHEASTERN INDIA
Objective: The objective of this study was to evaluate the disease prevalence and drug utilization pattern in the department of cardiology in a tertiary care hospital in northeastern India.Methods: Indoor case papers 112...
GENETIC DIVERSITY ASSESSED THROUGH RAPD MARKERS IN SYZYGIUM ALTERNIFOLIUM (WT) WALP
Objective: The present study has been carried out to evaluate the genetic diversity in a representative population of Syzigium alternifolium from six different accessions of chittoor district.Methods: Genomic DNA isolati...
FREEZE-DRIED POLYMERIC NASAL INSERTS FOR ANTIHYPERTENSIVE DRUG DELIVERY
Objective: The aim of this study was to investigate the ability of polymers to form verapamil HCl nasal inserts.Methods: Theinserts were prepared by lyophilization technique using bioadhesive polymers as chitosan, sodium...
ANTIOXIDANT ACTIVITIES OF SOME WILD EDIBLE PLANTS OF MEGHALAYA STATE IN INDIA AND EFFECT OF SOLVENT EXTRACTION SYSTEM
Objective: The antioxidant activities of some wild edible plants of different botanical families growing in the Meghalaya state, India e. g the bulb of Dioscorea bulbifera, the underground stem of Homalomena aromatica, f...