Green coffee bean hydroalcoholic extract accelerates wound healing in full-thickness wounds in rabbits
Journal Title: Traditional Medicine Research - Year 2020, Vol 5, Issue 6
Abstract
Background: Green coffee beans contain biochemical compounds including caffeine, chlorogenic acid, trigonelline, and diterpenoid alcohols. In traditional Iranian medicine, coffee bean powder is used for the treatment of wounds. Previous studies have shown that green coffee bean extract has a number of health benefits, including induction of weight loss, reduced blood pressure, and hepatoprotective and anti-Parkinson effects. The aim of this study was to investigate the wound-healing activity of green coffee bean extract on a full-thickness wound model. Methods: Full-thickness wounds of 20 × 20 mm were created on the back of New Zealand white rabbits. The animals were divided into 6 groups. Three concentrations of green coffee bean extract (5%, 10%, and 15% w/w) in a eucerin base were applied over the wounds in 3 test groups. One percent phenytoin cream was used in one group as a positive control. The rabbits of negative control and vehicle groups received no treatment and eucerin, respectively. For the evaluation of green coffee bean extract’s wound-healing effects, measures included: (1) wound-closure rate, by daily measuring of the wound surface area and calculating the reduction in area; (2) period of epithelialization, the number of days until the scab dropped from the wound; (3) hydroxyproline content, measured by a hydroxyproline assay kit; (4) lipid peroxidation, measured by a lipid peroxidation assay kit; and (5) histopathological state (hematoxylin-eosin stain) of wound-tissue samples on days 7 and 14 post-wounding. Results: The results of this study showed significantly enhanced wound-closure rate, shorter period of epithelialization (P < 0.01), increased hydroxyproline content (P < 0.001) and suppressed lipid peroxidation (P < 0.001 on day 14) of wounds on animals treated with the 10% green coffee bean extract compared to the negative control and eucerin-treated groups. Moreover, the therapeutic effects of 10% green coffee bean were significantly superior than those of phenytoin on enhancing wound-closure rate, decreasing period of epithelialization (P < 0.05), increasing hydroxyproline content (P < 0.001 on day 7 and P < 0.0001 on day 14) and suppressing lipid peroxidation (P < 0.0001 on day 14). In addition, histopathological study supported the wound healing activity of green coffee bean extract. Conclusion: Our results showed that green coffee bean extract has a potential for promoting wound healing, thus supporting its traditional use for this purpose.
Authors and Affiliations
Reza Shahriari,Pari Tamri,Amir Larki Harchegani,Alireza Nourian
Keywords
Related Articles
- EP ID EP690497
- DOI 10.12032/TMR20200603191
- Views 135
- Downloads 0
Long-term effect of Chinese herbal medicine Tianqi Capsule on the incidence of diabetes: an 8-year cohort study protocol
Background:The rapid growth of type 2 diabetes mellitus (T2DM) remains a big challenge for clinicians worldwide. Traditional Chinese medicine may bring a new approach for solving this problem. The Tianqi Capsule Diabetes...
A systematic summary of natural compounds in Radix Glycyrrhizae
Objective: To accumulate data from studies on the compounds identified from Gancao (Radix Glycyrrhizae, GC), and then systematically summarize and classify these compounds according to their structural characteristics. M...
Advances in research on the anticancer mechanism of the natural compound cucurbitacin from Cucurbitaceae plants: a review
Cucurbitacins are highly oxidized tetracyclic triterpenoids that are widely found in plants belonging to Cucurbitaceae family and exert various pharmacological effects. Many cucurbitacin derivatives are available, of whi...
Effect of Jianpi-yangwei decoction on gut fungi in the patients with gastric cancer undergoing chemotherapy
Background: Our previous study shows that the empirical formula of Chinese medicine Jianpi-yangwei decoction (JYD) can improve the quality of life in patients with gastric cancer undergoing chemotherapy by increasing ben...
Uncertainty profile for NIR analysis of tanshinone I content in tanshinone extract powders
A rapid, non-destructive and reliable NIR method was developed and validated for the quantification of tanshinone I content in Salvia miltiorrhiza extract. After spectral pretreatment, different variable selection method...