Growth Kinetics of Egyptian isolates of Trichomonas vaginalis: Possible Correlation to Clinical Presentation
Journal Title: The Egyptian Journal of Hospital Medicine - Year 2018, Vol 72, Issue 5
Abstract
<span>Background: </span><span>Trichomonas vaginalis </span><span>is the most common non-viral sexually transmitted infection (STI) in the world. Trichomoniasis is an important risk factor for herpes simplex type II infections, human immunodeficiency virus (HIV) acquisition and transmission, pelvic inflammatory disease and cervical neoplasia. Studying the variation in growth patterns of the parasite can be used for biological characterization of the parasites and might be related to the severity of clinical presentation.<br /> </span><span>Aim of the Work: </span><span>Study the growth kinetics of Egyptian </span><span>Trichomonas vaginalis </span><span>isolates in term of log phase, growth peaks reached, division rate and generation time of each isolate and correlation of such parameters with the clinical presentation of the patients, if any.<br /> </span><span>Material and Method: </span><span>The study was carried on 300 vaginal washouts collected from Egyptian women patients aged 20-45 years suspected for trichomoniasis. Positive samples for </span><span>Trichomonas vaginalis </span><span>were </span><span>cultured and sub cultured on modified Diamond’s medium. Growth kinetics for each isolate was done by </span><span>inoculated 10</span><span>4 </span><span>trophozoites/ml into 2 tubes containing 10 ml of sterile culture medium followed by counting the trophozoites every 24 hours over seven days using a hemocytometer. Growth curves were done for each isolate by plotting the parasites numbers against the time. Division rates and generation times were calculated for each isolate.<br /> </span><span>Results: </span><span>Out of the 300 collected samples, twelve were found positive for </span><span>Trichomonas vaginalis </span><span>trophozoite by wet mount examination and culture technique. Comparison of the growth kinetics of the twelve </span><span>Trichomonas vaginalis </span><span>isolates revealed a salient difference among all isolates after 48 to 96 hours. Isolates 1, 2, 6, 8 and 10 had a log phase of 48 hours. Isolates 4, 5, 7, 11 and 12 had a log phase of 72 hours. Isolates 3 and 9 had a log phase of 96 hours. The fast growing isolates of </span><span>Trichomonas vaginalis </span><span>reached maximum growth after 48 hours and the highest yield was observed in isolate 10 (150.25±3.13). Slowly growing isolates reached a maximum after 96 hours with the lowest yield in isolate 3 (40.5±21). Regarding the clinical presentation, isolates 1, 2, 3, 4, 5 and 11 showed mild discharge and congested cervix. Isolates 6, 7, 8, 9, 10, and 12 showed profuse discharge with varying clinical findings on gynecological examination. Isolate 10 had the most severe pathology with cervical erosion. Isolate 9, 12 showed bleeding during examination, isolate 8 showed vaginal ulcer while isolate 6 showed just erythema of the vagina.<br /> </span><span>Conclusion: </span><span>Comparison of the growth kinetics of the twelve </span><span>Trichomonas vaginalis </span><span>isolates revealed a salient difference among all isolates after 48 to 96 hours. Regarding the clinical presentation, no clear correlation was found between the growth kinetics and clinical presentation of the patients. Methods other than growth kinetics such as genotyping and determination of genetic variability are needed to verify the relation between isolates of </span><span>Trichomonas vaginalis </span><span>and clinical presentation.<br /> </span>
Authors and Affiliations
Eman Helal
Keywords
Related Articles
- EP ID EP584158
- DOI 10.12816/ejhm.2018.9493
- Views 116
- Downloads 0
Renal Stones among Adult Population in Arar City, Northern Saudi Arabia
<span>Background: </span><span>Renal stone is a major health problem with adverse medico-economic consequences. It cost the healthcare services a great deal of money without reaching any desired destiny. </span><span>Obj...
Timing of Renal Replacement Therapy and its impact on the Outcome of Acute Kidney Injury Patients
<strong>Background: </strong>Previous studies using Acute Kidney Injury Network (AKIN)/RIFLE criteria to classify early initiation of renal replacement therapy (RRT) have defined it as the therapy started in less severe...
Prevalence of Internal Hernia Post Laparoscopic Surgery
Background: Laparoscopic approach for colorectal resections is now very popular. Internal small bowel herniation (SBH) through a mesenteric defect has been recorded especially if the mesentery is not repa...
Dynamic Contrast-Enhanced Magnetic Resonance Imaging; A Useful Tool for Characterization and Assessment of Ovarian Masses
<strong>Objectives: </strong>To evaluate the diagnostic performance of dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) for the characterization of complex ovarian tumors, by using histologic findings as th...
Contact Lens-related Complications
<span>Aim of the work</span><span>: the contact lenses are widely used for management of refractive errors and cosmetic purposes as well. Their complications are rare and usually caused by in appropriate maintenance of t...