GSTP1, GSTM1, and GSTT1 polymorphisms in Tibetan mountaineers
Journal Title: Biology of Sport - Year 2006, Vol 23, Issue 3
Abstract
Exposure to high altitude can increase the level of reactive oxygen species (ROS) in human beings. Physical exercises or working can exacerbate the effects of high altitude and further cause free radical mediated oxidative tissue injury. The glutathione S-transferases (GSTs) can scavenge ROS. Variant genotypes of GSTs lead to the inactive or decreased form of the enzymes. We hypothesized that the polymorphisms within these genes may explain the interindividual variation in response to high altitude hypoxia. We have evaluated the polymorphisms of GSTP1, GSTM1 and GSTT1 genes in 86 excellent Tibetan mountaineers and in 90 sea-level Han Chinese were used as the control group. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were performed to genotype GSTP1 polymorphism in exon 5 (Ile105Val). Both GSTM1 and GSTT1 genotypes were determined by multiplex PCR. The result showed that GSTM1 null genotype was found in 60.5% of Tibetan mountaineers and in 54.4% of sea-level Han Chinese. No difference was observed in the frequency of polymorphic genotype for GSTM1 (×2=0.65, p=0.26), (OR=0.78; 95% CI:0.43-1.42). The frequency of GSTT1 null genotype was 36.1% among Tibetan mountaineers, 51.1% among Han Chinese. The difference was statistically significant (×2=4.06, p=0.031), (OR=1.86; 95% CI: 1.01-3.39). The proportion of GSTP1-105 mutant homozygote was significantly lower in the Tibetan mountaineers than in the control subjects (26.7% vs 44.4%) (×2=5.99, p=0.011).The OR for GSTP1-105 mutant genotype versus wild genotype was 2.19 (95% CI=1.16-4.13). These results suggest that GSTT1 and GSTP1-105 genotypes may be associated with the interindividual variation in response to high altitude hypoxia, and may be two new markers in gene screening for human adaptation to high altitude.
Authors and Affiliations
H Yan, X Sun, W Den, T Liu
Keywords
Related Articles
- EP ID EP58886
- DOI -
- Views 107
- Downloads 0
ANTHROPOMETRIC DETERMINANTS OF ROWING ERGOMETER PERFORMANCE IN PHYSICALLY INACTIVE COLLEGIATE FEMALES
The aim of the study was to evaluate anthropometric characteristics as determinants of 500 m rowing ergometer performance in physically inactive collegiate females. In this cross-sectional study, which included 196 colle...
Overrepresentation of the COL3A1 AA genotype in Polish skiers with anterior cruciate ligament injury
Although various intrinsic and extrinsic risk factors for anterior cruciate ligament (ACL) rupture have been identified, the exact aetiology of the injury is not yet fully understood. Type III collagen is an important fa...
Characteristics of the inspiratory muscle strength in the well-trained male and female athletes
Measurement of the maximal inspiratory mouth pressure (PImax) is a simple, reproducible, and non-invasive method frequently used for estimation of the inspiratory muscle strength. The aim of the study was to assess the P...
Spirulina does not decrease muscle damage nor oxdidative stress in cycling athletes with adequate nutritional status
The objective of this study was to assess the effect of Spirulina maxima on oxidative stress and muscle damage in cycling athletes subjected to high volume and intensity of training. Eighteen male athletes were randomly...
EFFECTS OF REPEATEDLY HEADING A SOCCER BALL ON SERUM LEVELS OF TWO NEUROTROPHIC FACTORS OF BRAIN TISSUE, BDNF AND NGF, IN PROFESSIONAL SOCCER PLAYERS
The present study determined the effects of heading training on serum nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) levels in soccer players. Seventeen professional level male soccer players (mea...