Guided Regeneration of Nerve Repair by Means of Local Administration of Insulin-Like Growth Factor I In Streptozotocin Induced Diabetes In Rats
Journal Title: International Journal of Surgery and Research (IJSR) - Year 2015, Vol 2, Issue 4
Abstract
The aim was to evaluate the effect of locally administered insulin-like growth factor (IGF I) on functional recovery of peripheral nerve in diabetic rats. Sixty male healthy white Wistar rats were divided into four experimental groups (n = 15), randomly: In transected group (TC), left sciatic nerve was transected and stumps were fixed in the adjacent muscle. In treatment group defect was bridged using a silicone conduit (SIL/IGF) and filled with 10 µL IGF I (100 ng/kg). In silicone graft group (SIL), the graft was filled with phosphate-buffered saline alone. In sham-operated group (SHAM), sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups of five animals each and regenerated nerve fibers were studied 4, 8 and 12 weeks after surgery. Behavioral testing, sciatic nerve functional study, gastrocnemius muscle mass and morphometric indices confirmed faster recovery of regenerated axons in SIL/IGF than in SIL group (p < 0.05). In immunohistochemistry, location of reactions to S-100 in SIL/IGF was clearly more positive than that in SIL group. When loaded in a silicone graft, IGF I accelerated and improved functional recovery and morphometric indices of sciatic nerve in diabetic rats.
Authors and Affiliations
Rahim Mohammadi
Keywords
Related Articles
- EP ID EP210510
- DOI 10.19070/2379-156X-150004
- Views 124
- Downloads 0
What is Acceptable; More Deaths Pre Transplant or More Deaths Post-Transplant. Share 35 Liver Allocation System
Since the adoption of this policy, more patients (28%) have been transplanted at high MELD scores (> 30) compared to transplant recipients in or prior to 2012 or [1738 (2012 actual) vs. 2128 (2014 actual)] [1]. A prelimi...
Colorectal Anastomotic Leakage: A new, Validated Rat Model
Background: Anastomotic leakage (AL) remains an important complication after colorectal surgery. Experimental research aims to find a solution to overcome this serious complication; however, no validated AL model exists...
Fibroadenoma of Ectopic Breast Tissue - A Case Report
Fibroadenomas of the breast are highly common among females whereas fibroadenomas of ectopic breast tissue, which can occur anywhere along the early ‘milk lines’ of breast development are a rare occurrence. We report the...
Combined Duodenal Atresia and Pure Esophageal Atresia
Combined duodenal atresia and pure esophageal atresia is a rare combination which has been reported only 13 times previously in the literature. Although this pair of congenital anomalies is amenable to current treatment...
Why is Postoperative Atrial Fibrillation Difficult to Prevent and Treat: Potential Roles of Unrecognized Magnesium Deficiency and Release of Ceramide and Platelet-Activating Factor
Heart failure is a major cause of morbidity and mortality whose costs impose staggering health-care costs and often lengthy hospitalizations. Post-operative atrial fibrillation (POAF) represents a leading cause for heart...