HaeIII Polymorphism of Growth Hormone (GH-1) Gene in Some Goat Breeds Reared in Turkey by Using PCR-RFLP Method
Journal Title: ISPEC Journal of Agricultural Sciences - Year 2023, Vol 7, Issue 3
Abstract
The purpose of this study is to determine HaeIII polymorphism in the exon 2 and 3 regions of the growth hormone -1 (GH-1) gene that regulates tissue growth and muscle development in six different goat breeds reared in Turkey. The HaeIII polymorphism in GH-1 gene (422 bp) was investigated by Restriction Fragment Length Polymorphism (PCR-RFLP) methodology in 303 goats including 52 Hair, 51 Angora, 50 Honamlı, 50 Halep, 50 Saanen and 50 heads of Kilis breeds. Two alleles (A and B) and 3 genotypes (AA, BB and AB) were identified in the study. A and B allele frequencies in Hair, Angora, Honamlı, Halep, Saanen and Kilis breeds were found to be 0.4038 and 0.5962, 0.4314 and 0.5686, 0.4600 and 0.5400, 0.4500 and 0.5500, 0.3800 and 0.6200, 0.5400 and 0.4600, respectively. AA, AB and BB genotype frequencies were found to be 00.019, 0.769 and 0.212 (P<0.05), 0.235, 0.392 and 0.373 (P>0.05), 0.080, 0.760 and 0.160 (P<0.05), 0.060, 0.780 and 0.160 (P<0.05), 0.160, 0.440 and 0.400 (P>0.05), 0.160, 0.760 and 0.080 (P<0.05), respectively. When all breeds were taken into consideration, A and B allel frequencies were found to be 0.4439 and 0.5561, and AA, AB and BB genotype frequencies were found to be 0.119, 0.650 and 0.231 (P>0.05). As a result, it was determined that goat population had high heterozygosity (0.494) in general and was not in Hardy-Weinberg equilibrium in terms of HaeIII polymorphism in the exon 2 and 3 regions of the GH-1 gene (P>0.05).
Authors and Affiliations
Mahamat Ali AMINE OUCHAR, Birol DAĞ, İbrahim AYTEKİN
Keywords
Related Articles
- EP ID EP724089
- DOI https://doi.org/10.5281/zenodo.8373425
- Views 65
- Downloads 0
Biochemical and Molecular Evaluation of Some Tomato Hybrids in Salt Stress At The Growth Stage
Growing stages of plants are the most sensitive phase to abiotic stress which leads to decrease in yield. The object of this study was to evaluate the biochemical and molecular properties of seven tomato cultivars and to...
Determination of Botanical Composition, Rangeland Status and Health of Elazıg Province Karakocan District Basyurt Village Rangeland
This study was carried out to determine the botanical composition, rangeland status and health of Elazığ province Karakoçan district Başyurt village rangeland. 41 plant species belonging to 15 families were determined in...
Generation Z's Perspectives on E-Commerce in Animal Products After The Covid-19 Pandemic (I)
The introduction of curfews in many countries with the COVID-19 epidemic in 2020 has led to changes in consumer behavior and reshaping of the marketing and distribution structure. In this context, this study investigated...
The Effect of Various Additives Addition to Barley-Vetch Mixtures on Silage Quality Characteristics and In vitro Digestibility
In this study, it was aimed to determine the effects of adding different levels of molasses and fructose syrup to barley-vetch mixtures, one of the roughage sources produced as a second product in cotton fields in Şanlıu...
Milk Composition Traits of Hamdani Crossbreed Sheep Raised Under Extensive Management
This study aimed to reveal the milk composition traits of Hamdani crossbred sheep (Hcs) raised under extensive conditions. A total of 96 sheep milk samples were collected during the early lactation stage. Selected ewes d...