Heme Oxygenase-1 Expresion in Gastric Mucosa and Liver after Burns: Preliminary Immunohistochemical Study
Journal Title: Journal of Interdisciplinary Histopathology - Year 2013, Vol 1, Issue 5
Abstract
Objective: Generation of reactive oxygen metabolites (ROM) and oxidative stress are crucial in pathogenesis of thermal skin injury. Heat shock proteins such as HSP32 known as heme oxygenase-1 (HO-1) possesses antioxidant, anti-inflammatory, and vasodilative properties and play an important role in the protecting of tissues from several stresses. In this immunohistochemical study, we have assessed the constitutive expression of HO-1 in normal gastric mucosa and liver and tested the hypothesis that its expression in these tissues is upregulated in thermal skin injury characterized by increased production of ROMs and other pro-inflammatory mediators. We used malondialdehyde (MDA) as a marker of oxidative stress and tumor necrosis factor-alpha (TNF-α) as a marker of inflammatory response in burn rat model. Methods: HO-1 expression in formalin-fixed sections was assessed by peroxidase anti-peroxidase immunohistochemistry using a polyclonal rabbit anti-HO-1 as primary antibody. TNF-α in plasma was quantified using enzyme-linked immunosorbent assay (ELISA) kit. Spectrometric method was used for plasma and tissue MDA assay. Results: Immunohistochemical staining revealed HO-1 expression in all groups. Staining scores for HO-1 in endothelial cells in both gastric mucosa and liver were increased with 32% (p <0.001) and 51 % (p< 0.001), compared to that of controls. The levels of MDA in the gastric mucosa were increased by 43% (p<0.05) and in liver in 126% (p<0.001). Plasma concentration of TNF-α was also elevated by 111 % (p<0.001) and MDA level in plasma was increased by 37% (p<0.05). Conclusion: Our data suggest that HO-1 induction following burn is an adaptive response which can protect gastric mucosal and liver against further oxidative damage. HO-1 system may represent a target and an effective and cooperative strategy to intervene in the protection against inflammatory processes and oxidative tissue injury.
Authors and Affiliations
Ganka Bekyarova, Maria Tzaneva, Minka Hristova
Keywords
Related Articles
- EP ID EP109837
- DOI 10.5455/jihp.20130417103023
- Views 113
- Downloads 0
Cytomegalovirus endometritis in a non-immunosuppressed patient presenting as recurrent pyometra: A case report
Cytomegalovirus infection of the female genital tract is a rare entity. We present a case of 70-year-old woman who came with recurrent pyometra but was otherwise in good health. Hysterectomy was done, and the endometrium...
Giant Congenital Cerebriform Melanocytic Nevus of the Scalp in Adult
Congenital melanocytic nevi are defined as benign nevomelanocytic proliferation present at birth. They are rare lesions and occur in approximately 1% of new-borns and are usually classified according to their size. We re...
Heme Oxygenase-1 Expresion in Gastric Mucosa and Liver after Burns: Preliminary Immunohistochemical Study
Objective: Generation of reactive oxygen metabolites (ROM) and oxidative stress are crucial in pathogenesis of thermal skin injury. Heat shock proteins such as HSP32 known as heme oxygenase-1 (HO-1) possesses antioxidant...
Histopathologic Effect of Prenatal Topiramate Exposure on Rat Cerebral Cortex and Hippocampus
Objective: Topiramate is a recent antiepileptic agent approved for treatment of seizure in adults, children and during pregnancy. No much available reports concerning topiramate teratogenic potential in humans. This coul...
Schistosomiasis: A rare cause of acute appendicitis
Schistosomiasis is a water-borne trematode infestation and is one of the most widespread parasitic diseases in the world. Schistosomiasis can affect any organ, but is rare in the appendix. In this paper, the authors repo...