Hepatitis B Virus DNA in Sera of Apparently Healthy Carriers Blood Donors with Anti-Hbc Total Positive
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 1
Abstract
Objective this study was attempt to molecular estimation of Hepatitis-B Virus DNA (HBV-DNA) in sera of apparently healthy carriers blood donors with anti-HBc Total positive alone and discussed the reasons that lead to disappearance of HBsAg in their sera. As well as evaluation of the routine screening method of HBV infection among blood donors by ELISA assay. Methods A total of 100 sera samples were collected from HBsAg-negative blood donors who were attended to the blood bank in Baghdad and examined for anti-HBc IgM, anti-HBc Total and anti-HBsAg. Thereafter samples with anti-HBc Total positive alone were separated and underwent molecular investigation for the presence of HBV-DNA by PCR technique. Results Out of 100 samples 9 (9 %) had anti-HBc Total positive and 0 % anti-HBc IgM negative. Out of 9 anti-HBc Total positive 6 (66.6 %) with anti-HBs positivity and 3 (33.33 %) were negative to anti-HBs. Furthermore, HBV DNA among HBsAg negative and anti-HBc Total positive alone was investigated. One case 11.11 % of the latter group was detecting positive for HBV- DNA. Conclusion HBV-DNA is present in sera of blood donors with HBsAg negative, anti-HBc Total positive and anti-HBs negative.
Authors and Affiliations
Thaer Kadhim Fayyadh, Fuying Ma
Keywords
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- EP ID EP588872
- DOI 10.9790/0853-160103138142
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