Hepatoprotective Activity and Inhibitory Effect of Flavonoid –Rich Extract of Brysocarpus Coccineus Leaves on Mitochondrial Membrane Permeability Transition Pore
Journal Title: ASIAN JOURNAL OF NATURAL & APPLIED SCIENCES - Year 2014, Vol 3, Issue 3
Abstract
The hepatoprotective activity of flavonoid –rich extract of Brysocarpus Coccineus leaves, a medicinal plant with anti-tumour ,anti-inflammatory and analgesic property was assessed in sodium arsenite- intoxicated rats,and its effects on Mitochondrial Membrane Permeability Transition (MMPT) Pore which when opened could lead to the release of Cytochrome C, a point of no return for Apoptosis to take place, was investigated in-vitro .Results showed that sodium arsenite intoxication caused significant ( P< 0.05) increases in serum cholesterol level and activities of Alanine Aminotransferase (ALT) Aspartate Aminotransferase (AST) and Gamma glutamyl transferase (γGT). The elevation of the levels of these enzymes could be suggestive of an early liver injury. Interestingly, flavonoids –rich extracts, treated with sodium arsenite ameliorated these effects as these parameters were restored significantly (P<0.05) nearly to their control levels thus suggests possible hepatoprotective activity of the extracts. Investigation of the flavonoid –rich extract on MMPT pore in-vitro showed that calcium ions induced the opening of MMPT pore significantly (P<0.05) in rat liver mitochondria while spermine inhibited calcium-induced opening of the pore, indicating that the mitochondria were intact ab-initio. The results further revealed that inhibitory effects of the extract on the pore opening (200, 600,1000,1400, and 1800µg/ml) were 68.00,72.00, 83.20, 84.20 and 87.70% respectively. In this regard the highest degree of inhibition (87.70%) by the extract at 1800µg/ml compared favourably with that of spermine (86.70%), the standard inhibitor. The behaviour of the extract may not be unconnected with its ability to interact with the pore components making it impossible for the mega channel to be assembled. The inhibition of the MMPT pore by the extract may represent an effective therapeutic approach in several pathological conditions which require reduced rate of apoptosis .For chemotherapy further elucidation of the structure of the bioactive agent is required.
Authors and Affiliations
Olaniyi T. Adedosu, Adejoke T. Oyedeji, Temitope Iwaku, Adeola F. Ehigie
Keywords
Related Articles
- EP ID EP163574
- DOI -
- Views 116
- Downloads 0
EFFECT OF WHEAT BRAN CONSUMPTION ON SERUM LIPID PROFILE OF HYPERCHOLESTEROLEMIA PATIENTS RESIDENCE IN HOLLY MAKAH
Background: Hypercholesterolemia is a metabolic disorder resembling a major risk factor for cardiovascular diseases. It is associated with hypertension, atherosclerosis, diabetes and hypertriglyceredemias. Therefore, it...
MEDICINAL UTILIZATION OF HERBS FOR THE TREATMENT OF ERECTILE DYSFUNCTION IN LERE LOCAL GOVERNMENT AREA OF KADUNA STATE, NIGERIA
This paper examined the indigenous uses of medicinal plants for the treatment of erectile dysfunction in Lere Local Government Area of Kaduna State, Nigeria. One hundred (100) respondents were purposively selecte...
Machine Learning and Statistical Based Investigations on Competitor Analysis – Certain Novel Discovered Facts
The paper entails certain discovered facts related to concept learning , artificial neural modeling of customer based approach of identifying competitors. Statistical based investigations have also been carried out based...
Investigation of Socio-Cultural Factors in Shaping Local Perceptions of Climate Chanage: A Case Study in NakornRatchasima, Thailand
There is a worldwide recognition that climate change is the most serious environmental threat to humanity in our time. Although the extreme natural events continue to occur, climate change issues are widely misunderstood...
SPECTRAL ANALYSIS MODEL OF SIMULATED N-TEAM INTERACTING DECISION MAKERS WITH BOUNDED RATIONALITY CONSTRAINTS
The spectral analysis model was derived to forecast performance of interacting decision makers, based on the simulated mode that was adopted for the model of N-Teams of interacting decision maker (DM) with bounded ration...