Hepcidin, Selenium and Superoxide Dismutase in Oxidative Stress and in Dialysis Patients
Journal Title: Open Access Journal of Urology & Nephrology - Year 2017, Vol 2, Issue 1
Abstract
Chronic kidney diseases (CKDs) are characterized by complex changes in cellular metabolism, leading to oxidative stress (OS), i.e. increased production of oxidative radicals (OR), which can play a key role in a number of clinical complications of this pathology. Trace elements selenium (Se), copper (Cu) and zinc (Zn) play a major role in the antioxidant defense system. The antioxidant enzymes are superoxide dismutases (SODs), catalases (CATs) and glutathione peroxidases (GSHPxs). We evaluated serum hepcidin, plasma selenium concentrations and the superoxide dismutase activity in 59 CKD patients on hemodialysis. Patients were monitored at University Hospital “Aleksandrovska”, Clinic of Dialysis Treatment and Clinic of Nephrology. The results were compared with a matched by sex and age control group. Serum hepcidin and SOD activity were determined by ELISA method.Plasma selenium concentration was examined with the help of AAS (Perkin-Elmer). We determined CBC (using hematology analyzer ADVIA 2120, Siemens Healthcare) and biochemical parameters (on automatic analyzer Dimension RxL MAX, Siemens Healthcare) of all participants. The serum iron and TIBC levels were determined by AAS (Perkin-Elmer). For statistical analysis of the results we used Student's paired t-test and Pearson's correlation. We found a statistically significant higher serum hepcidin levels in CKD patients on chronic dialysis (356.6 μg/L ± 52.5 μg/L) compared to the control group (19.4 μg/L ± 2.7 μg/L; P<0.001). Plasma selenium levels and SOD activity were decreased in CKD patients on on chronic dialysis (422.6 nmol/L ± 51.1 nmol/L; 45.3 μg/mL ± 6.8 μg/mL) compared to the control group (847.5 nmol/L ± 75.4 nmol/L; 101.6 μg/mL ± 10.9 μg/mL; P<0.001). The presence of anemic syndrome and oxidative stress are indisputable pathological conditions accompanying chronic kidney diseases. A significant percentage of patients on dialysis are with abnormal levels of certain important trace elements - Zn, Se, Cu, and magnesium and one recently discovered regulator of iron metabolism - hepcidin, and between all of the following conditions are found interconnections and influences.
Authors and Affiliations
Manolov V
Keywords
Related Articles
- EP ID EP496982
- DOI 10.23880/oajun-16000116
- Views 70
- Downloads 0
Coagulum, Tumor, Pyonephrosis?
Pyonephrosis is the accumulation of pus in the renal pelvis and calyces. It represents a medical emergency and usually develops as a complication of infected hydronephrosis. If clinically suspected, pyonephrosis can be d...
A Randomised Prospective Study Analysing the Effects of Lowering Serum Uric Acid in Chronic Kidney Disease (CKD) Progression and Cardiovascular Outcomes
Uric acid is an independent risk factor for the development and progression of kidney disease. In last two decades number of observational studies has examined the potential link between management of hyperuricemia with...
Residual Urine Measurement in Children with Voiding Disorders: Comparison between Ultrasonographic Bladder Scanning and Catheterization
Postvoid residual urine (PVR) measurement with a bladder scanning portable ultrasonographic device is an easy and non invasive method. Its use has been wide spread in men and women however its accuracy and reliability in...
Current Trends in Use of Holmium-Yag Laser in Percutaneous Nephrolithotomy
The EAU Guidelines recommend percutaneous nephrolithotomy (PCNL) as the treatment of choice for renal stones >2cm, because of the higher stone free rate compared to extracorporeal shock wave lithotripsy (ESWL) and the lo...
Utilization of the Charlson Comorbidity Score to Predict 10-Year Survival in Renal Transplant Recipients
Purpose: The Charlson Comorbidity Score has been demonstrated to predict mortality in prostate cancer patients. Our novel objective was to compare the 10-year observed mortality with expected mortality based on pre-opera...