HiCrome UTI Agar : An Alternative To Conventional Media For Presumptive Identification And Isolation Of Uropathogens
Journal Title: IJSR-International Journal Of Scientific Research - Year 2016, Vol 5, Issue 12
Abstract
The increase in resistance of uropathogens to antimicrobial agents, especially in hospitalized patients, demands rapid identification of the pathogen. Current detection methods including culture and identification are time-consuming. The chromogenic media offers simultaneous presumptive identification of gram-positive and gram-negative bacteria and yeasts on a single medium by means of distinct colony colours produced by reactions of genus- or species-specific enzymes with a suitable chromogenic substrate. The present study was undertaken to validate the usefulness of HiCrome UTI agar as a primary urine culture medium for its rate of isolation and presumptive identification of uropathogens in comparison to CLED agar, BA and MA agar. This study included 1500 midstream and/or catheter-catch urine samples from clinically suspected UTI patients of different age and sex groups. HiCrome UTI agar, MacConkey agar , Blood agar and CLED agar media were obtained as a dehydrated powder from HiMedia laboratories. Urine culture was done on above said media and presumptive identification of bacterial growth was done Out of total 1500 urine samples,552 urine samples showed significant growth of a single isolate and 13 urine samples showed mixed growth of two organisms and 50% samples were sterile. Among all the bacterial isolates E.coli were maximum in number (n=195), followed by Klebsiella spp. (n=81).Staphylococcus aureus and Candida spp. constitute 12.97 % and 7.61% of the total urinary isolates, respectively. Maximum number of urinary isolates was presumptively identified on Hicrome UTI agar (88.06 %) and there is no significant difference in the rate of presumptively identification from CLED agar and Blood agar and MacConkey agar in combination (62.97% and 61.24, respectively). Presumptively identification of mixed growth of organisms on Hicrome UTI agar was 100%, but CLED agar, Blood agar and MacConkey agar in combination failed to presumptively identify 100% mixed growth of organisms. The overall findings of this study suggests that though expensive, chromogenic media like HiCrome UTI Agar media, is an acceptable alternative to traditional media for the isolation of urinary pathogens.
Authors and Affiliations
Pradnya Atmaram Jadhav, Shahriar B. Roushani
Keywords
Related Articles
- EP ID EP344855
- DOI -
- Views 58
- Downloads 0
CLINICAL & HISTOPATHOLOGICAL ANALYSIS OF 50 CASES OF TERATOMA IN A TEACHING HOSPITAL
Teratoma constitutes 20% of all ovarian neoplasms .The origin of teratomas has been a matter of interest, speculation, and dispute for centuries. OBJECTIVE: The aim of the study is to characterize the clinical and detail...
DRUG DELIVERY THROUGH DRUG ELUTING STENTS -A REVIEW
Drug eluting stents were first developed in an attempt to prevent or address the issue of restenosis. Drug eluting stents consists of three parts namely, a stent platform, a coating of polymer that binds the drug to the...
MULTIPLE ENDOCRINE NEOPLASIA TYPE 2B SYNDROME: A RARE CASE PRESENTATION
Multiple endocrine neoplasia Type 2b is a rare syndrome caused by mutations in RET proto oncogene. It is a rare entity, which is found 1 in 30,000 people. We report a case of 35-year-old male patient who presented with 1...
THE ROLE OF HEART TYPE FATTY ACID BINDING PROTEIN IN THE EARLY DIAGNOSIS OF ST SEGMENT ELEVATION MYOCARDIAL INFARCTION
INTRODUCTION Ischemic heart disease is one of the leading causes of death all over the world and hence early diagnosis of acute coronary syndrome is crucial in prognosis. Heart type fatty acid binding protein (H-FABP) is...
TO STUDY 25 (OH) D LEVELS IN PATIENTS WITH NON ALCOHOLIC FATTY LIVER DISEASE
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is a common cause of chronic liver disease and its increasing incidence has been well documented from Asian countries. Diabetes mellitus (DM), obesity, hyperlipidemia...