HIGH FREQUENCY SOMATIC EMBRYOGENESIS FROM COTYLEDONARY EXPLANTS OF INDIGOFERA TINCTORIA LINN.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2018, Vol 6, Issue 7
Abstract
Protocol was standardized for the rapid clonal propagation through high frequency indirect somatic embryogenesis from the cotyledonary explants of a medicinally and commercially important plant, I. tinctoria Linn. Maximum embryogenic callus was induced from explants cultured on MS medium amended with 2, 4-D (0.5 mgl-1) and BA (0.5 mgl-1). Abnormal somatic embryos were developed from NAA and BA containing cultures. Among different additives or amino acids used, L-glutamine enhanced maximum high frequency embryogenic callus formation. Embryogenic calluses, when subcultured to ? MS basal medium induced maximum embryo conversion. BA or KIN along with MS or ? MS medium also enhanced the development of embryos. Maturation and rooting of somatic embryos took place in ? MS solid medium enriched with 5% sucrose. Plantlets derived from somatic embryos were hardened in sugar free liquid ? MS basal medium. Subsequent transfer of hardened plantlets to vermiculite and then to soil recorded maximum survival.
Authors and Affiliations
A. S. Rubin jose, g. M. Nair.
Keywords
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- EP ID EP366814
- DOI 10.21474/IJAR01/7462
- Views 60
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