High-intensity interval training increases mitochondria biogenesis in adipose tissue and improves insulin resistance in high fat diet-induced obese rat
Journal Title: International Journal of Applied Exercise Physiology - Year 2019, Vol 8, Issue 1
Abstract
The purpose of this study was to investigate the effects of high intensity interval training (HIIT) on PGC-1α and mtDNA of subcutaneous adipose tissue and insulin resistance in obese male rats. After inducing obesity with high fat diet(for 10 weeks), eight rats from the high-fat diet group (O) and eight rats of the standard dietary group (C) were sacrificed and other obese rats were randomly divided into two groups: obesity control (OC, n=8) and high intensity interval training (HIIT, n=8). The HIIT protocol includes 10 bouts of 4-minute activity with equivalent intensity of 85-90% vo2max and 2 minute active rest periods for 12 weeks and 5 sessions per week. Western Blot method was used to measure PGC-1α and real time PCR was used to measure. Obesity induced by a high-fat diet resulted in a significant reduction in protein levels of PGC-1α [0.25±0.13 vs. 1] and insignificant reduction in gene expression of mtDNA [0.80±0.02 vs. 1]. In contrast, HIIT resulted in a significant increase in protein levels of PGC-1α [1.64±0.40 vs. 0.29±0.03] and gene expression of mtDNA [4.92±0.59 vs. 0.89±0.02]. In addition to, HIIT resulted in a significant decrease in serum levels of insulin [1.49±0.15 vs. 2.14±0.43 ng/ml] and glucose [111.16±4.07 vs. 154.66±13.21 mg/dl] and improved insulin resistance insulin [1.96±0.20 vs. 3.89±0.54]. It seems likely that HIIT result in increase of adipose tissue mitochondrial biogenesis in obesity which may be involved in improving insulin resistance.
Authors and Affiliations
Mousa Khalafi, Hamid Mohebbi, Pouran Karimi
Keywords
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- EP ID EP572157
- DOI 10.30472/ijaep.v8i1.366
- Views 43
- Downloads 0
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