High Performance Liquid Chromatographic Analysis of Almotriptan Malate in Bulk and Tablets
Journal Title: Advanced Pharmaceutical Bulletin - Year 2013, Vol 3, Issue 1
Abstract
Purpose: A simple RP-HPLC method has been developed and validated for the determination of almotriptan malate (ATM) in bulk and tablets. Methods: Chromatographic separation of ATM was achieved by using a Thermo Scientific C18 column. A Mobile phase containing a mixture of methanol, water and acetic acid (4:8:0.1 v/v) was pumped at the flow rate of 1 mL/min. Detection was performed at 227 nm. According to ICH guidelines, the method was validated. Results: The calibration curve was linear in the concentration range 5–60 μg/mL for the ATM with regression coefficient 0.9999. The method was precise with RSD <1.2%. Excellent recoveries of 99.60 - 100.80% proved the accuracy of the method. The limits of detection and quantification were found to be 0.025 and 0.075 μg/mL, respectively. Conclusion: The method was successfully applied for the quantification of ATM in tablets with acceptable accuracy and precision.
Authors and Affiliations
Petikam lavudu, Avula Prameela Rani, Chepuri Divya, Chandra Bala Sekaran
Keywords
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- EP ID EP146254
- DOI 10.5681/apb.2013.030
- Views 77
- Downloads 0
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