High Performance Liquid Chromatographic-UV Method for Determination of Atorvastatin Calcium in Pharmaceutical Formulations
Journal Title: Journal of New Developments in Chemistry - Year 2017, Vol 1, Issue 3
Abstract
The effectiveness of atorvastatin calcium in lowering cholesterol is dose-related. It is available in 10, 20, 40, and 80 mg film coated tablets. In order to ensure quality, safety and efficacy of tablets in formulations, the objective of this presented work was to develop a new high performance liquid chromatographic-UV method for quantitation of active atorvastatin calcium in pharmaceutical formulations. The method was based on reversed-phase high performance liquid chromatographic-UV separation of atorvastatin at detection wavelength of 246 nm using Acclaim 120 C18 reversed phase LC column (5 mm, 250×4.6 mm) with mobile phase of acetonitrile-dichloromethane-acetic acid (68.6: 30.6: 0.8 v/v/v) at a flow rate of 1.0 mL min-1 at 25°C. Different variables affecting chromatographic separation were carefully studied and optimized. The study results provided chromatogram of atorvastatin with retention time of 2.68 min. The calibration curve was linear over the concentration range of 15-300 mg mL-1. No interference was observed from common pharmaceutical excipients present in dosage forms. The proposed method was successfully applied to the determination of atorvastatin calcium in pharmaceutical formulations and proved to be significantly not different with reference method. The proposed can be used as an alternate method for routine quality control analysis of active atorvastatin in research, hospitals and pharmaceutical laboratories.
Authors and Affiliations
Syed Najmul Hejaz Azmi, Amna Khalifa Al-Mamari, Khalifa Al-Mamari, Manal Rashied Al-Fazari
Keywords
Related Articles
- EP ID EP322473
- DOI 10.14302/issn.2377-2549.jndc-17-1439
- Views 159
- Downloads 0
Molecular Structure and Vibrational Analysis of 2-(4-methoxyphenyl)-2, 3-Dihydro-1H-Perimidine using Density Functional Theory
The compound 2-(4-methoxyphenyl)-2, 3-dihydro-1H-perimidine (MPDP) was synthesized. The molecular structure and its functional groups were characterized with the help of Fourier Transform Infrared: FTIR/ Fourier Transfor...
Preparation Physico-Chemical Characterization and Catalytic Applications of Polymer Anchored Dimethylglyoxime Copper (II) Metal Complex
Oxidation of 1-Proponol, 1-butanol 1-Hexanol and benzyl alcohol using chloromethylated polystyrene (6% Cross link) DMG (Dimethylglyoxime) copper complex or Poly(S-DVB)-DMG-M Where M= Cu) as catalyst and tert.butylhydrope...
An Optical Chemical Sensor for Determination of Nickel in Water and Hydrogen Peroxide Samples
Application of nickel in different industries has been developed and so contamination of natural water is a great concern due to its potentially toxic effects on living beings. Therefore, fast monitoring of Ni2+ in aqueo...
Development of Poly-ε-Caprolactone Based Nanoadjuvant for Effective Vaccination Against Tuberculosis
The aim of the study was to synthesize sub-100nm poly-ε-caprolactone nanoparticles (PCL NP), load them with the mycobacterial protein, ESAT 6 and study the resulting immune responses in CD4+ and CD8+ T cells when incubat...
Vibrational Spectral Analysis and First Order Hyperpolarizability Calculations on (E)-N′-(furan-2-yl methylene) Nicotinohydrazide
Vibrational spectral analysis and first order hyperpolarizability calculations on (E)-N′-(furan-2- ylmethylene) nicotinohydrazide (F2CNH), a novel, organic, hydrozone Schiff base compound was synthesized and its structur...