High Performance Liquid Chromatography Assay of AntiMalarial Quinine Sulfate Utilizing Isocratic Solvent Conditions
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2018, Vol 8, Issue 9
Abstract
A method to assay quinine sulfate, which is utilized for the treatment of Plasmodium falciparum, is presented using isocratic high performance liquid chromatography (HPLC). Elution of the analyteis detected with ultraviolet light detector, set at 222 nm. The stock solution of quinine sulfate was prepared in solvent conditions that consisted of 64 % ethanol (v/v) and 36 % water (v/v), at a concentration of 1.3155 x 10-2 molar. The test samples of quinine sulfate that were injected into HPLC instrument were mainly in a solvent that consisted of 95% (v/v) water and 5 % ethanol (v/v). The solvent utilized for column of the HPLC instrument was 5 % ethanol, 1 % glacial acetic acid (v/v), and 94 % water (v/v). A limit of detection (LOD) was found to be 2.2628 x 10-5 molar and the limit of quantitation (LOQ) was found to be 7.542 x 10-5 molar. A standard curve presented showed a coefficient of determination of R2 = 0.9972, which indicates that the model describes 99.72% variance in the dependent variable (peak area) that is predictable from the independent variable (molar concentration). The Pearson r correlation coefficient of this standard curve is 0.9986, indicating very high positive correlation. Quinine sulfate is assayed from various matrixes that are utilized in the formulation of this important drug, including5% glucose, 0.9% sodium chloride, water, and cellulose for tablet formulation. The determination of the drug quinine sulfate is consistent and accurate utilizing isocratic conditions with HPLC.
Authors and Affiliations
Ronald Bartzatt, Mai Han Cassandra Nguyen, Gunner Brantley, Aysha Hussain, Keerthi Shaik, Purnima Gajmer, Zohal Alizai, Mara Bullock
Keywords
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