HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD FOR ESTIMATION OF PANTOPRAZOLE IN INJECTION
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 8
Abstract
A simple and sensitive high performance thin layer chromatography (HPTLC) method has been developed for the quantitative estimation of pantoprazole in its single component injection formulation (40 mg). Pantoprazole chromatographed on silica gel 60 F254 TLC plate using toluene: ethyl acetate: methanol: acetic acid (7:2:1:0.1 v/v/v/v) as mobile phase. Pantoprazole showed Rf value 0.40 + 0.005 and scanned at 290 nm using a camag TLC scanner 3. The method was validated in terms of linearity (50 – 800 ng/spot), precision (intra-day variation, 1.28 to 2.40% and inter-day variation, 2.40 to 3.62%), accuracy (98.16 to 100.5%) and specificity. The limit of detection and limit of quantification for pantoprazole were found to be 8.45 ng/spot and 25.60 ng/spot, respectively. The developed method was successfully used for the assay of pantoprazole injection formulation. The method was found to be simple, sensitive, specific, accurate and precise and can be used for the routine quality control testing of pantoprazole in injection dosage form.
Authors and Affiliations
Satish Patel
Keywords
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