Histological Studies on Heteracris littoralis (Rambur) Treated with Silica Nano-particles / Challenger Formulation
Journal Title: Asian Journal of Biology - Year 2017, Vol 2, Issue 3
Abstract
Aim: Local diatomaceous earth (Silica nano-particles) and commercial pesticide (Challenger 36% SC) formulation was tested against alfalfa grasshopper, Heteracris littoralis (Rambur, 1838) (Orthopetra: Acrididae) under laboratory conditions. Study Design: Histological study for alimentary tract using light microscope. Place and Duration of Study: Department of Pests and Plant Protection, National Research Centre, Egypt- 2016-2017. Methodology: Fourth instar nymphs of H. littoralis were taken from laboratory culture reared on semi-artificial diet [1] for the experiments. The nymphs were fed on diet mixed with 1% concentration of Silica nano-particles/Challenger formulation. The tissue specimens of the alimentary canal were dissected in 0.9% NaCl solution and fixed in Bouin's solution for 24 hours [2] then dehydrated in ascending alcoholic series and cleared in Xylen for few seconds, and then specimens were infiltrated in three changes of paraffin wax each lasted 20 minutes. With Ehrlich's acid haematoxylin and alcoholic eosin. The stained sections were dehydrated, cleared and mounted using D.P.X. For microscope examination. Results: The Silica nano-particles induced great irregularity or deformity in the alimentary tract, that leading finally to death. All parts of the alimentary tract were affected by silica treatment (fore-gut, mid-gut and associated gastric caeca and hind-gut). Conclusion: Histological changes were observed as a result of Silica effect on the enzymes and the physiology of the treated insect. So, it could be concluded that Silica nano-particles may be used through the integrated pest management programme against H. littoralis by spray on the plants or as a food bait traps.
Authors and Affiliations
Aziza F. Sharaby, Amany R. Ebeid, Mohamed A. Gesraha
Keywords
Related Articles
- EP ID EP337840
- DOI 10.9734/AJOB/2017/33780
- Views 51
- Downloads 0
Some New Information on Ants’ Cemeteries Organization
Aim: We aimed to explain how ants manage to deposit nestmate corpses outside their nest. Methodology: We first summarized a previous study of ours dealing with the subject, and reported others carried out in the meantime...
Toxicological Effects of Different Concentrations of Hypo Bleach on the Mortality of African Clariid Mud Catfish (Clarias gariepinus) Fingerlings
Aim: The study was aimed at evaluating the mortality and LC50 of Clarias gariepinus exposed to different concentrations of hypo bleach. Methodology: Thirty (30) fingerlings were used for each aquarium exposed to four (4)...
Hepatitis B Virus and Hepatitis C Virus in Hemodialysis Patients: A Prevalence Study from Dialysis Centers in El-Beyda and Almarj- Libya
Background: Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections represent significant public health issues globally. These infection is a lead cause of morbidity and mortality among hemodialysis (HD) patients....
Homestead Plant Diversity at the Ship Breaking Areas of Bangladesh
Unplanned ship breaking at the Sitakund coast in Chittagong, Bangladesh is contaminating the surrounding vegetation mainly through the discharge of heavy metals from the discarded ship paints. Therefore, the present stud...
Seroprevalence of Hepatitis B Virus among Pregnant Women Attending Ante-natal Clinic at General Hospital Argungu, Kebbi State Nigeria
Hepatitis B virus infection is caused by Hepatitis B virus, and the virus can be transmitted from infected mother to her new born child during pregnancy. This research work was aimed at determining the prevalence of Hepa...