HISTOLOGICAL STUDY OF THE LIVER OF RATS CONSUMING GENETICALLY MODIFIED SOYBEAN, PROCESSED WITH HERBICIDE "ROUNDUP"
Journal Title: Вісник Одеського національного університету. Біологія - Year 2018, Vol 23, Issue 2
Abstract
Introduction. In last years the genetically modified plants have been widely used in agriculture, as well as in the food industry. The use of “Roundup” herbicide in the world increases every year, so there is a great warning as to its impact on the health of humans and animals. Purpose. The aim of the paper is to evaluate histopathological changes in the liver of two generations of rats under the influence of “Roundup” herbicide and transgenic glyphosate-resistant soya treated with this herbicide. Results. The subject of the study were the male rats of the Vistar line at the age of 4 months weighing 180-200 g. Experimental animals were grouped into 5 groups of 14 rats in each (7 females and 7 males). Group I - intact animals (kept on a standard vivarium diet); Group II - animals, which had up to 20-26% of the standard diet replaced with traditional soya; Group III - rats up with to 20-26% of the standard diet replaced with genetically modified soybeans not treated with herbicide “Roundup”; Group ІV - rats, whose ration contained up to 20-26% of genetically modified soybean, treated with herbicide “Roundup”; Group V - rats that received used “Roundup” herbicide with drinkable water at a concentration (0.003 μg / kg of animal weight). After 42 days of keeping to a respective diet rates were mated and after 22-25 days the next generation was obtained (F1). After giving birth to offspring, all females continued to receive the same diet and the herbicide with water as (F1) generation. At the age of 12 months, the rats were decapitated and sampling of liver fragments for histological test was performed. In the liver of the first generation rats, fed with genetically modified soya processed with the herbicide, discomplexation of lamellar structure, as well as cloudy degeneration of hepatocytes. The same condition was observed in rats that consumed the herbicide with drinkable water. Degenerative changes of hepatocytes aggravated in the second generation. Conclusions. Histological analysis of the liver of rats of all experimental groups of two generations showed that the most dystrophic changes are observed in the liver of rats which ate genetically modified soya treated with the herbicide and of rats which consumed the herbicide with drinking water. In the next generation, an increase in dystrophic changes in hepatocytes is observed. Microscopic changes indicate the energy load and intensification of detoxification processes by these cells.
Authors and Affiliations
I. V. Chorna, G. V. Dronik, I. S. Davydenko
Keywords
Related Articles
- EP ID EP504266
- DOI 10.18524/2077-1746.2018.2(43).147004
- Views 90
- Downloads 0
THE GENOTYPES OF MODERN MYRONIVSKY VARIETIES OF WINTER WHEAT FOR Ppd-A1, Ppd-B1, Ppd-D1 GENES AND THEIR SENSITIVITY TO PHOTOPERIOD
Problem. The system of photoperiodic response genes Ppd-1 affects the speed of winter wheat growth. Dominant alleles (a) of Ppd genes reduce the sensitivity to daylength and shorten the time period before heading, while...
THE STRUCTURE OF THE GREEN PLANTATIONS IN BOGDAN KHMELNITSKY PARK AND PROSPECTS OF THEIR DEVELOPMENT (KRYVYI RIH, DNIPROPETROVSK REGION)
Introduction. Kryvyi Rih is located in the steppe zone of Ukraine and covers the area of 4.75 thousand km2; 94.9 % of this area being classified as anthropogenically transformed territories. In conditions of arid climate...
EFFECTS OF THE DWARFING GENE ALLELES ON COLEOPTILE OF BREAD WHEAT LENGTH UNDER OSMOTIC STRESS CONDITIONS
One of the critical periods of plant response to drought is the germination stage. Deviations from the optimal conditions of growth in the period of germination negatively affect the development of wheat plants in the fo...
EFFECTS OF CHROMOSOMES ELYMUS SIBIRICUS ON AGRONOMICAL TRAITS IN SUPPLEMENTED-DISOMNO LINES OF WINTER WHEAT
There were evaluated the differences between the line and grade «Obriy» created on its base disomno-supplemented line «O27-2», which was bearing the alien signs of resistance to leaf rust and the leaf trichomes on amfipl...
DEPENDENCE OF THE PHENOTYPES OF THE MYTILUS GALLOPROVINCIALIS (LAMARCK, 1819) ON THE DEPTH OF GROUND IN THE ODESA BAY OF THE BLACK SEA
Introduction. The Black Sea mussel Mytilus galloprovincialis (Lamarck, 1819) is one of the dominant species of macrozoobenthos and fouling of the Black Sea. It occurs on rocks and stones, on shell, sand and muddy ground,...