Histomorphometric Analysis of Angiogenesis using CD31 Immunomarker and Mast Cell Density in Oral Premalignant and Malignant Lesions: A Pilot Study
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 1
Abstract
Introduction: Mast cells have been implicated in promoting angiogenesis in malignant tumors of lung, oesophagus and breast, but there are few studies on Oral Squamous Cell Carcinomas (OSCC). Most oral squamous cell carcinomas arise from pre-existing precancerous lesions exhibiting epithelial dysplasia. Aim: The present pilot study attempts to compare Mast Cell Density (MCD), Microvessel Density (MVD), Microvessel Area (MVA) histomorphometrically between normal buccal mucosa, severe epithelial dysplasia and OSCC and to correlate the role of mast cells and angiogenesis in tumor progression. Material and Methods: The retrospective study was conducted on eight cases of OSCC, eight cases of severe epithelial dysplasia and five cases of normal buccal mucosa. Immunohistochemical staining with anti CD–31, to demonstrate angiogenesis and toluidine blue staining for mast cells were employed. MVA, MVD and MCD were calculated using the measurement tools of the image analysis software and compared between the groups. One way ANOVA (Analysis of Variance) was used for comparing the parameter for multiple groups followed by Games Howell test. To assess the relationship between micro vessel density and mast cell density, Karl Pearson’s correlation was used. Results: MCD and MVD increased with disease progression and were statistically higher in OSCC than in severe epithelial dysplasia and normal buccal mucosa (p<0.001). MVA increased from normal to severe dysplasia and decreased from dysplasia to OSCC, may be due to revascularization of tumor tissue. A positive correlation was observed between MCD and MVD in OSCC and dysplasia, though were not statistically significant. Conclusion: These findings suggest that mast cells may up regulate angiogenesis in OSCC. MCD and MVD may be used as indicators for disease progression.
Authors and Affiliations
M Jyothsna, Kiran Kumar
Keywords
Related Articles
- EP ID EP328888
- DOI 10.7860/JCDR/2017/23870.9179
- Views 169
- Downloads 0
Factor Structure of Schizotypal Personality in India
ABSTRACT Introduction: Schizotypal personality represents genetic underpinning of schizophrenia spectrum disorders; hence, it provides conceptual models for understanding psychosis as well as a scheme for high-risk group...
Ureteric Obstruction due to Aberrant Vessel Masquerading as Retrocaval Ureter
Abstract Aberrant vessels, although rare, may extrinsically compress ureter which on imaging may masquerade as retrocaval ureter. The exact cause of obstruction in such atypical cases can be confirmed intraoperatively....
Light Microscopic Features of Type II Dens Invaginatus in A Deciduous Mandibular Molar
Images in Medicine
Assessment of Lipid Peroxidation and Antioxidant Status in Vanillic Acid Treated 7,12-Dimethylbenz[a] anthracene Induced Hamster Buccal Pouch Carcinogenesis
Introduction: Vanillic acid, a naturally occurring bioactive substance, possesses diverse pharmacological potential including free radical scavenging and anticancer properties. Excessive generation of Reactive Oxygen Spe...
Evaluation of Liver Stiffness in Healthy Individuals by Sonoelastography
Introduction: Successfully detecting and managing Liver Fibrosis (LF) at an early phase can significantly improve the prognosis of patients in clinical practice; therefore, accurately assessing the degree of fibrosis whe...