Histopathological and Immunohistochemical Study of the Toxicity of UVB-Irradiation on Rabbit's Cornea: Possible Antioxidant Role of Trehalose
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2014, Vol 4, Issue 16
Abstract
Aims: This Study aimed to evaluate toxic changes that might occur in rabbit cornea after UVB exposure and possible protective role of Trehalose. Study Design: Eighteen adult white female rabbits were divided into three groups, six rabbits for each one. Group Ι received buffered saline (negative control), Group ΙΙ irradiated by UVB (positive control) and Group ΙΙI irradiated by UVB with concomitant application of Trehalose eye drops. Place and Duration of Study: Department of Pharmacology, and Department of Histopathology, Umm AL Qura University (UQU), KSA, between April 2012 and May 2012. Methodology: Eighteen rabbits were divided into three equal groups. Group Ι received buffered saline (0.9%), Group ΙΙ Only the cornea was irradiated by UVB, and Group ΙΙI were treated (by dropping trehalose three drops in each eye, six times daily) after irradiated with UVB. Two weeks after treatment, the excised corneas were employed for histopathological and immunohistochemical examinations. Results: UVB exposure induced cell death and thinning of the corneal epithelium, while the irradiated epithelium was preserved well morphologically with concomitant application of Trehalose eye drops. The irradiated corneal epithelium was significantly (P =0.026) thicker than eyes treated with Ultraviolet and Trehalose eye drops. Apoptotic cells were significantly (P =0.001) reduced in negative control and Trehalose -treated eyes than positive control eyes. Trehalose was showed to prevent oxidative stress, accelerated corneal healing, restored corneal transparency and prevent corneal neovascularization in irradiated corneas. Caspase-3, was strong significantly expressed (P<0.5) in the corneal epithelium two weeks after irradiation and corneal neovascularization was evident. Conclusion: Trehalose prevents apoptosis after the UVB irradiated cornea, so topical Trehalose administration may be a candidate treatment to prevent the damages by UVB irradiation with wide applications in clinical toxicology.
Authors and Affiliations
Ibrahim S. Elgendy, Ragia M. Hegazy
Keywords
Related Articles
- EP ID EP349456
- DOI 10.9734/BJMMR/2014/8354
- Views 65
- Downloads 0
Loss of Erythrocyte Deformability under Oxidative Stress is caused by Protein Oxidation with Consequent Degradation Rather than by Lipid Peroxidation
Aims: Loss of erythrocyte deformability under oxidative stress is poorly understood. The present study aimed to determine which of the detrimental effects of oxidant stress, namely, lipid peroxidation or protein degradat...
Aggressive Angiomyxoma of the Vulva: Libyan Female Case Report
Aims and Background: Aggressive angiomyxoma is rare tumor of pelvic and perineal organs, occurring usually in women of reproductive age, and carrying a high tendency to local infiltration and relapse. Most literature abo...
Occupational Risk Factors for Musculoskeletal Disorders among Operation Room Nurses at Cairo University Hospitals
Aims: To assess the occupational risk factors that contribute to the occurrence of work- related musculoskeletal disorders among Operation Room (OR) nurses and MSDs relation to biomarker c-telopeptide of type II collag...
Schizophrenia and Its Association with Biological and Environmental Factors: A Community Based Study
Aims: The aim of this study was to examine the proportion of probable schizophrenia in Arab population visiting primary health care centers and investigate its associated biologic and environmental factors. Study Design...
Can 0.025-inch Guidewire VisiGlideTM become a Standard in the ERCP-related Procedures? VIP Study
Aims: With the progress of development of 0.025-inch guidewire (GW), various treatments with 0.025-inch GW have become possible. To date, however, there has been no multicenter cooperative prospective study using 0.025-i...