Histopathological evaluation of the effect of platelet-rich fibrin on canine cutaneous incisional wound healing
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2013, Vol 5, Issue 2
Abstract
Platelets are a natural source of growth factors and cytokines that promote wound healing, and platelet-rich fibrin contains concentrated growth factors. Growth factors in platelet-rich fibrin may promote wound repair. In this study the effect of platelet-rich fibrin was evaluated in a dog model of cutaneous incisional wounds using histology and semi-quantitative evaluation. A pair of ten-centimeter full-thickness parallel linear cutaneous wounds were created on the backs of 15 dogs on the both sides of vertebral column. On the left side the platelet-rich fibrin clot was applied to the edges of the wound (Treatment group) and the right side received nothing (Control group). All wounds were then closed with 3-0 non-absorbable Nylon suture. The dogs were divided into three groups of five dogs. The wound tissues were sampled by electrosurgery in group one after 3 days, group two after 7 days and group three after 14 days post surgery. For each specimen, histopathological examination and semi-quantitative evaluation was performed by light microscopy using Hematoxylin & Eosin and Massonâs trichrome staining. The results demonstrated that platelet-rich fibrin improved and accelerated cutaneous incisional wound healing.
Authors and Affiliations
Majid Khanzadeh Alishahi,Davoud Kazemi,Darioush Mohajeri,Hasan Mofidpoor,Amir Afkhami Goli,Mohamad Ali Khanzadeh Alishahi,
Keywords
Related Articles
- EP ID EP722491
- DOI -
- Views 44
- Downloads 1
Therapeutic effects of pomegranate (Punica granatum) pith and carpellary membrane extract on lead-induced toxicity in rats
Pomegranate is an ancient edible fruit with various pharmaceutical bioactivities such as antioxidant, lipid regulation and anti-inflammation. In this study the effectiveness of pomegranate pith and carpellary membrane (P...
Treatment of critical-sized calvarial non-union defect via collagen-polyglycolic acid scaffold loading with simvastatin in rabbits
The aim of the present study is to investigate the effects of a sustained-release simvastatin in collagen-polyglycolic acid scaffold on bone formation in the rabbit calvarial critical-sized defect. This study was carried...
Isolation and identification of Brachyspira pilosicoli from laying hens flocks, using conventional culture and molecular methods in Mashhad, Iran
Avian intestinal spirochaetosis (AIS) is an intestinal infection caused by anaerobic spirochaetes of the genus Brachyspira, including B. pilosocoli. The purpose of this study was isolation and identification of B. pilos...
Preparation and in vitro evaluation of chitosan-based films for the sustained delivery of enrofloxacin
The implantable drug products are developed mainly to sustain the drug release. This study was conducted to formulate and evaluate cross-linked films of chitosan/β-glycerophosphate (β-GP) for the sustained delivery of...
Recovery effects of pomegranate seed powder on the testes following cadmium poisoning in Japanese quail (Coturnix japonica); a stereological and lipid peroxidation study
This study aimed to investigate the effects of pomegranate seed powder on cadmium-poisoned testicular tissue in Japanese quail. A total of 270 day-old Japanese quail chicks were assigned to six treatment groups, control...