HOTAIR expression in newly diagnosed Acute Lymphoblastic Leukemia pateints
Journal Title: Khoon - Year 2018, Vol 15, Issue 1
Abstract
HOTAIR expression in newly diagnosed Acute Lymphoblastic Leukemia pateints Fallah P.1, Molaie M.2, Ehsan Heidari A.3, Soleimani M.4, Jahedi Zargar M.1, Arefian E.5, Madani T.6, Asadi M.3, Poopak B.7 1Allied-Health Faculty, Alborz University of Medical Sciences, Karaj, Iran 2Stem Cell Technology Research Center, Tehran, Iran 3Faculty of Medical Sciences, Alborz University of Medical Sciences, Karaj, Iran 4Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran 5Biology School of Tehran University, Tehran, Iran 6Peyvand Clinical & Specialty Laboratory, Tehran, Iran 7Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran Abstract Background and Objectives Acute lymphoblastic leukemia (ALL) is the most common malignancy in childhood. The prevalence of ALL is 1 to 4/7 per 100,000 population every year world-wide. Long non-coding RNAs (LncRNAs) are a group of non-coding RNAs involved in many biological processes, such as epigenetic changes, regulation of mRNA transcription and translation. Identification of their function can be helpful to understand the pathophysiology and treatment of ALL. One of the important LncRNAs in hematopoiesis regulation is HOTAIR (LncRNA HOX transcript antisense RNA). The purpose of this study was to determine the expression of HOTAIR LncRNA in newly diagnosed B-ALL patients. Materials and Methods In this cross-sectional descriptive study, 40 newly diagnosed B-ALL patients as well as 15 healthy individuals as control were given 1mL of bone marrow specimen with EDTA anticoagulant. RNA (patients and control) was extracted and Real-time quantitative PCR was used to examine the expression of LncRNA HOTAIR in ALL patients. The results was analyzed by REST 2009. Results The findings showed that expression of HOTAIR in patients with Acute Lymphoblastic Leukemia type B increased significantly as compared to healthy controls (p ˂ 0.05). Conclusions In conclusion, the increase expression of HOTAIR in acute lymphoblastic leukemia can be attributed to potential role of LncRNA HOTAIR in pathophysiology of the disease. Key words: Hematopoiesis, LncRNAs, Lymphocytic Leukemia، Acute Received: 22 Aug 2017 Accepted: 13 Feb 2018 Correspondence: Poopak B., PhD of Hematology and Blood Banking. Assistant Professor of Islamic Azad University, Tehran Medical Sciences Branch. P.O.Box: 19395-1495, Tehran, Iran. Tel: (+9821) 22264144; Fax: (+9821) 22264144 E-mail: bpoopak@gmail.com
Authors and Affiliations
P. Fallah, M. Molaie, A. Ehsan Heidari, E. Arefian, T. Madani, B. Poopak
Keywords
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