HPLC Analytical Method Development and Validation Studies of Ondansetron and Rabeprazole.
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2012, Vol 1, Issue 3
Abstract
Objective: Present investigation involves development and validation of chromatographic method for ondansetron and rabeprazole estimation by HPLC. Methods: The present work describes a validated reverse phase high performance liquid chromatographic method for simultaneous estimation of Rabeprazole and Ondansetron in bulk and pharmaceutical dosage form. Chromatography was performed on a XTerra RP column C18 (150 mm x 4.6 mm i.d., 5 μm) column with mobile phase containing Buffer (phosphate buffer pH 5.5): Water: Methanol (30: 10: 60 v/v/v). The flow rate was 0.6 ml/min and the eluent was monitored at 274 nm. Rabeprazole and Ondansetron were studied for their estimation by RP-HPLC method development and the method was also studied for validation parameters. Results: The selected chromatographic conditions were found to effectively separate Rabeprazole (RT-5.919 min) and Ondansetron (RT- 4.382 min). Linearity for Rabeprazole and Ondansetron were found in the range of 20-100 μg/ml. The values obtained of LODs were 3.06 and 0.06 μg/ml; LOQs were 9.90 and 0.21 μg/ml for Rabeprazole and Ondansetron, respectively. The proposed method was found to be fast, accurate, precise, reproducible and rugged and can be used for simultaneous analysis of Rabeprazole and Ondansetron in combined pharmaceutical formulations. Conclusion: Developed HPLC method was conveniently used for the estimation of ondansetron and rabeprazole in bulk and formulations and the results obtained in this study were uniform, sensitive and reproducible within the limits. Therefore the method is suitable for its intended use for routine analysis of both drug candidates.
Authors and Affiliations
Udaya Krishna T| Department of Pharmaceutical Analysis and Pharmaceutics1, V.L. College of Pharmacy, Raichur, Karnataka., Jitendra K| Department of Pharmaceutical Analysis and Pharmaceutics1, V.L. College of Pharmacy, Raichur, Karnataka., Putta Rajesh Kumar*| Department of Pharmaceutical Analysis and Pharmaceutics1, V.L. College of Pharmacy, Raichur, Karnataka., Salahuddin Md| Department of Pharmaceutical Analysis and Pharmaceutics1, V.L. College of Pharmacy, Raichur, Karnataka., Shanta Kumar S. M| Department of Pharmaceutical Analysis and Pharmaceutics1, V.L. College of Pharmacy, Raichur, Karnataka.
Keywords
Related Articles
- EP ID EP1577
- DOI -
- Views 412
- Downloads 25
Knowledge, Awareness and Disposal Practice for Unused Medications among the Students of the Private University of Bangldesh
Medicines play an important role in treating many conditions and diseases, but when they are no longer needed it’s important to dispose of them properly to avoid harm to others, so knowledge and practice of unused medica...
HELLP SYNDROME: A BRIEF REVIEW
HELLP is an abbreviation of hemolysis (H), elevated liver enzyme (EL), and low platelet count (LP) which is a serious complication in pregnancy and also described as a severe form of preeclampsia and which is also associ...
BRINE SHRIMP LETHALITY TEST AND ANTI-PROLIFERATION TEST AGAINST HUMAN CANCER-ORIGIN CELL LINES USING ETHANOLIC AND WATER EXTRACTS OF SELAGINELLADOEDERLEINII HIERON
Most of the properties of herbal products claimed in traditional use are based on traditional knowhow and/or empirical observations. Support from scientific evaluation and validation is thus crucial. Selaginella doederle...
ROLE OF BHALLATAKARASAYANA IN CANCER
An integrated approach is required in the management of cancer by using the knowledge gained through various scientific developments. Thousands of traditional compounds are being screened worldwide to validate their effi...
Comparative study of Lipid Profile of Different Age Group and Gender Patients Attending OPD of Chitwan Medical College Teaching Hospital.
Information regarding lipid profile of different age group in the inhabitants of Chitwan is still very scanty. Lipid Profile tests were used to assess risk of heart disease in all age group populations. Lipid Profile we...