HPLC method for simultaneous determination of oxytocin and clorobutanol in injectable solutions
Journal Title: Medicamentul Veterinar / Veterinary Drug - Year 2013, Vol 7, Issue 2
Abstract
The objective of this work was to establish and validate a HPLC method with UV detection for simultaneous determination of oxytocin and clorobutanol in veterinary injectable formulations. The method is based on European Pharmacopoeia monograph for oxytocin concentrated solution. Chromatographic separation was achieved on a Syn Chropack RP100 C18 column (250 x 4,6mm, 5 μm) with a mobile phase consisting of solutia A: sodium dihydrogen phosphate buffer 0,13 M and solution B: acetonitrile-water (1:1, v/v) with gradient elution (30 % B for 1 min, 30 % B to 60 % B in 30 min, return to initial concentration and echilibration for 15 min before the following injection), at a flowrate of 1 ml/min and detection at 220 nm. The retention times for oxytocin and clorobutanol were about 15 min and 26 min respectively. The degradation products of oxytocin eluted at retention times smaller than 14 min. The resolution between oxytocin and the nearest impurity fulfilled the USP monograph requirements of at least 1,5. The linearity of the method has been settled from 2,5 UI/ml to 20 UI/ml for oxytocin and 1,25 mg/ml to 10 mg/ml for clorobutanol. In these ranges the corelation coefficients were higher than 0,9950. The method allows the separation of oxytocin from degradation products and clorobutanol and could be applicable to quality control of injectable products containing oxytocin and clorobutanol. The method was validated in terms of selectivity, linearity, precision and accuracy.
Authors and Affiliations
Ana Csuma, Despina Niţă, Luciana Paraschiv
Keywords
Related Articles
- EP ID EP99052
- DOI -
- Views 138
- Downloads 0
Mathematical modeling for determining the potency of bovine tuberculin purified protein derivative (P.P.D.)
Bovine tuberculin purified protein derivative (bovine tuberculin PPD) is a preparation obtained from the heat-treated products of growth and lysis of Mycobacterium bovis, strain AN5, purified and solubilized into a liqui...
Preliminary experimental research on acute toxicity of Vernonia kotschyana extracts in mice
Vernonia kotschyana Sch. Beep. ex Walp (Asteraceae) is used in traditional medicine of Mali to treat gastric diseases and gastro-duodenal ulcer. Three extracts were obtained from vegetal material, represented by roots of...
Synergistic interaction between trimetazidine and ketoprophen in mice
Recent studies have demonstrated the antinociceptive, anti-inflammatory, and gastric protective effects of trimetazidine (TDZ) on various models in rats. The present study proposes to demonstrate the antinociceptive acti...
The Analytical method (HPLC) used for identification, assay of triclabendazole, related substances and preservatives used in finished product Tricladem 5, in SC Delos Impex 96 SRL
Because, triclabendazole is an active pharmaceutical ingredient without compendial monography (European Pharmacopoeia, United Stated Pharmacopoeia) in SC Delos Impex ’96 SRL, the API identification, assay, related...
Forced degradation of Romprogestin 1% for HPLC-method development and drug stability assessment
Forced degradation is an analytical technique applied to a drug product in order to simulate conditions more severe than those in accelerated stability studies. Active substances may suffer degradation during storage ove...